Evaluation of several techniques to modify denatured muscle tissue to obtain a scaffold for peripheral nerve regeneration
| Autor: | Wilfred F. A. den Dunnen, Peter H. Robinson, Marcel F. Meek, J.M. Schakenraad |
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| Přispěvatelé: | University of Groningen |
| Rok vydání: | 1999 |
| Předmět: |
Male
Muscle tissue Protein Denaturation Pathology medicine.medical_specialty Time Factors Biophysics Nerve guidance conduit Biocompatible Materials Bioengineering Biology Transplantation Autologous GUIDE Biomaterials Extracellular matrix Laminin Materials Testing medicine Animals Peripheral Nerves Rats Wistar Axon Muscle Skeletal nerve regeneration VEIN CONDUITS Nerve Transfer LONG-TERM EVALUATION REPAIR cryo-scanning Regeneration (biology) DEFECTS Immunohistochemistry Axons denatured muscle Rats LAMININ medicine.anatomical_structure Mechanics of Materials Peripheral nervous system Microscopy Electron Scanning SURVIVAL Ceramics and Composites biology.protein RAT Basal lamina Collagen |
| Zdroj: | Biomaterials, 20(5), 401-408. ELSEVIER SCI LTD University of Groningen |
| ISSN: | 0142-9612 |
| DOI: | 10.1016/s0142-9612(98)00178-1 |
| Popis: | The aim of this study was to (1) evaluate the effect of several preparation techniques of denatured muscle tissue to obtain an open three-dimensional structure, and (2) test if this scaffold is suitable for peripheral nerve regeneration. Four samples (A-D) of muscle tissue specimens were evaluated using light microscopy, immunohistochemistry and cryo-scanning electron microscopy. Sample C showed the most open extracellular matrix, whilst collagen type IV and laminin (in the basal lamina) could still be observed using immunohistochemistry. An in vivo pilot study showed that the first signs of functional nerve recovery and axon regeneration could be observed after 3 weeks of implantation. We conclude that sample C has the most open structure and leads to good nerve regeneration and functional nerve recovery. (C) 1999 Published by Elsevier Science Ltd. All rights reserved. |
| Databáze: | OpenAIRE |
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