Allovahlkampfia spelaea Causing Keratitis in Humans
| Autor: | Seiki Kobayashi, Mohammed E.M. Tolba, Hanan El Deek Mohamed, Enas Abdelhameed Mahmoud Huseein, Sumio Sugano, Haiam Mohamed Mahmoud Farrag, Jun Suzuki, Tarek Ahmed Mohamed Ali |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Eye Infections Acanthamoeba Human pathogen Biochemistry 18S ribosomal RNA chemistry.chemical_compound 0302 clinical medicine Gene duplication Medicine and Health Sciences Amoebas Phylogeny Protozoans Mammals lcsh:Public aspects of medicine Animal Models Amebiasis Nucleic acids Infectious Diseases Ribosomal RNA Vertebrates Rabbits Anatomy Research Article Cell biology Cellular structures and organelles food.ingredient lcsh:Arctic medicine. Tropical medicine lcsh:RC955-962 Biology Research and Analysis Methods DNA Ribosomal Microbiology Keratitis Amoeba (genus) 03 medical and health sciences Model Organisms food Ocular System Parasite Groups parasitic diseases Genetics RNA Ribosomal 18S medicine Animals Humans Trophozoites Non-coding RNA Organisms Gene Amplification Public Health Environmental and Occupational Health Biology and Life Sciences lcsh:RA1-1270 DNA Protozoan biology.organism_classification medicine.disease Parasitic Protozoans Ophthalmology Disease Models Animal 030104 developmental biology chemistry Amniotes 030221 ophthalmology & optometry RNA Eyes Parasitology Ribosomes Head Apicomplexa DNA |
| Zdroj: | PLoS Neglected Tropical Diseases, Vol 10, Iss 7, p e0004841 (2016) PLoS Neglected Tropical Diseases |
| ISSN: | 1935-2735 1935-2727 |
| Popis: | Background Free-living amoebae are present worldwide. They can survive in different environment causing human diseases in some instances. Acanthamoeba sp. is known for causing sight-threatening keratitis in humans. Free-living amoeba keratitis is more common in developing countries. Amoebae of family Vahlkampfiidae are rarely reported to cause such affections. A new genus, Allovahlkampfia spelaea was recently identified from caves with no data about pathogenicity in humans. We tried to identify the causative free-living amoeba in a case of keratitis in an Egyptian patient using morphological and molecular techniques. Methods Pathogenic amoebae were culture using monoxenic culture system. Identification through morphological features and 18S ribosomal RNA subunit DNA amplification and sequencing was done. Pathogenicity to laboratory rabbits and ability to produce keratitis were assessed experimentally. Results Allovahlkampfia spelaea was identified as a cause of human keratitis. Whole sequence of 18S ribosomal subunit DNA was sequenced and assembled. The Egyptian strain was closely related to SK1 strain isolated in Slovenia. The ability to induce keratitis was confirmed using animal model. Conclusions This the first time to report Allovahlkampfia spelaea as a human pathogen. Combining both molecular and morphological identification is critical to correctly diagnose amoebae causing keratitis in humans. Use of different pairs of primers and sequencing amplified DNA is needed to prevent misdiagnosis. Author Summary Free-living amoebae are present worldwide. Some species are known to cause chronic keratitis in human. Amoebic chronic keratitis is sight-threatening disease occurring in both developing and well-developed countries. Allovahlkampfia spelaea is a newly discovered free-living amoeba. We report the first human case of chronic keratitis due to that amoeba. For correct identification, both morphological and molecular techniques should be combined. |
| Databáze: | OpenAIRE |
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