Combinatorial regulation of stilbene synthase genes by WRKY and MYB transcription factors in grapevine (Vitis vinifera L.)
| Autor: | Jochen Bogs, Darren C. J. Wong, Margherita Lucchin, Gianni Barcaccia, Alessandro Vannozzi, Janine Höll, Ibrahim Hmmam, Tobias Ziegler, José Tomás Matus, Ian B. Dry |
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| Přispěvatelé: | Fondazione Cariparo, Università degli Studi di Padova |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Physiology Arabidopsis Network Plant Science Biology Gene co-expression Resveratrol Vitis vinifera Genes Plant 01 natural sciences Transcriptome 03 medical and health sciences Gene Expression Regulation Plant Stress Physiological MYB Gene Regulatory Networks Vitis RNA Messenger Gene Transcription factor Phylogeny Regulation of gene expression Genetics Effector Reproducibility of Results Promoter Cell Biology General Medicine WRKY protein domain 030104 developmental biology Acyltransferases 010606 plant biology & botany Transcription Factors |
| Zdroj: | Recercat. Dipósit de la Recerca de Catalunya instname Dipòsit Digital de Documents de la UAB Universitat Autònoma de Barcelona Digital.CSIC. Repositorio Institucional del CSIC Recercat: Dipósit de la Recerca de Catalunya Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) Digital.CSIC: Repositorio Institucional del CSIC Consejo Superior de Investigaciones Científicas (CSIC) |
| Popis: | Stilbene synthase (STS) is the key enzyme leading to the biosynthesis of resveratrol. Recently we reported two R2R3-MYB transcription factor (TF) genes that regulate the stilbene biosynthetic pathway in grapevine: VviMYB14 and VviMYB15. These genes are strongly co-expressed with STS genes under a range of stress and developmental conditions, in agreement with the specific activation of STS promoters by these TFs. Genome-wide gene co-expression analysis using two separate transcriptome compendia based on microarray and RNA sequencing data revealed that WRKY TFs were the top TF family correlated with STS genes. On the basis of correlation frequency, four WRKY genes, namely VviWRKY03, VviWRKY24, VviWRKY43 and VviWRKY53, were further shortlisted and functionally validated. Expression analyses under both unstressed and stressed conditions, together with promoter–luciferase reporter assays, suggested different hierarchies for these TFs in the regulation of the stilbene biosynthetic pathway. In particular, VviWRKY24 seems to act as a singular effector in the activation of the VviSTS29 promoter, while VviWRKY03 acts through a combinatorial effect with VviMYB14, suggesting that these two regulators may interact at the protein level as previously reported in other species. This work was supported by the Cariparo Foundation [Cariparo Starting Grants] and the Department of Agronomy, Food, Natural Resources, Animals, and Environment (DAFNAE), University of Padova. |
| Databáze: | OpenAIRE |
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