Development of C-TILDA: A modified TILDA method for reservoir quantification in long term treated patients infected with subtype C HIV-1
| Autor: | Vanessa D'Urbano, Tokameh Mahmoudi, Isabella Bon, Maria Carla Re, Charles A. Boucher, Annelies Verbon, Jeroen J. A. van Kampen, Casper Rokx, Elisa De Crignis, Alessia Bertoldi, Giorgio Gallinella, Rob A. Gruters, Leonardo Calza |
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| Přispěvatelé: | Internal Medicine, Medical Microbiology & Infectious Diseases, Virology, Biochemistry, Bertoldi A., D'Urbano V., Bon I., Verbon A., Rokx C., Boucher C., van Kampen J.J.A., Gruters R.A., Gallinella G., Calza L., Mahmoudi T., De Crignis E., Re M.C. |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
CD4-Positive T-Lymphocytes Serial dilution Sustained Virologic Response 030106 microbiology Human immunodeficiency virus (HIV) CD4-CD8 Ratio HIV Infections Biology medicine.disease_cause Antiviral Agents Sensitivity and Specificity Virus HIV Testing 03 medical and health sciences SDG 3 - Good Health and Well-being Virology HIV subtype C medicine Humans Cell stimulation Gene Messenger RNA Hiv epidemiology HIV Viral Load Virus Latency 030104 developmental biology Reservoir quantification DNA Viral HIV-1 TILDA CD8 |
| Zdroj: | Journal of Virological Methods, 276:113778. Elsevier |
| ISSN: | 1879-0984 0166-0934 |
| Popis: | A better characterization of the HIV reservoir is pivotal for the development of effective eradication strategies. Accurate quantification of the latent reservoir remains challenging. Starting from a regular blood draw, the Tat/Rev induced limiting dilution assay (TILDA) combines serial dilution of CD4+ T cells with a PCR-based detection of HIV-1 spliced mRNA produced upon cell stimulation. Here we adapted the original protocol for HIV-1 subtype B to detect tat/rev mRNAs transcribed from reactivated latently infected cells in long term suppressed patients infected with HIV-1 subtype C. Given the heterogeneity of global HIV epidemiology, it is pivotal to develop assays with optimal performances also in patients infected with non-B subtypes. We observed that, in these patients infected with subtype C virus, the HIV reservoir quantified by TILDA correlates with both the time of virological suppression and CD4/CD8 ratio. |
| Databáze: | OpenAIRE |
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