Evidence that specific high mannose structures directly regulate multiple cellular activities
| Autor: | Jean M. Decker, Anne P. Sherblom, Andrew Muchmore, Neeraja Sathyamoorthy |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Swine
T cell Clinical Biochemistry Guinea Pigs Molecular Sequence Data Mannose Oligosaccharides Biology Dinoprostone Pentose Phosphate Pathway chemistry.chemical_compound Immune system Mucoproteins Antigen In vivo Uromodulin medicine Animals Humans Hexose Hypersensitivity Delayed Molecular Biology Glycoproteins chemistry.chemical_classification Immunity Cellular Cell Biology General Medicine In vitro Stimulation Chemical medicine.anatomical_structure Biochemistry chemistry Carbohydrate Sequence Leukocytes Mononuclear Swine Miniature Glycoprotein Immunocompetence Interleukin-1 |
| Zdroj: | Molecular and cellular biochemistry. 102(2) |
| ISSN: | 0300-8177 |
| Popis: | Previous studies have demonstrated that much of the immunomodulatory activity of the glycoprotein uromodulin can be attributed to attached oligosaccharides. Structural studies of isolated and purified saccharides derived from uromodulin suggest that the structure Man6GlcNAc2-asn can inhibit in vitro assays of antigen driven T cell proliferation. Based on these observations, we isolated a series of high mannose glycopeptides from a variety of natural sources and tested them for biological activity in a number of assays. We found that purified mannose rich glycopeptides are able to activate the hexose monophosphate (HMP) shunt, induce prostaglandin synthesis, and directly stimulate IL-1 synthesis. These in vitro effects appear to have in vivo counterparts. Thus in a species-restricted fashion, high mannose compounds are able to directly activate a delayed mononuclear cell infiltrate after intradermal injection. Our data suggest that specific mannose oligosaccharides may activate as well as inhibit cellular immune responses at several different levels. These findings support the hypothesis that specific saccharide structures could participate in the physiologic regulation of the immune response. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink