Effects of CpG-B ODN on the protein expression profile of swine PBMC

Autor: Cheng-Chin Kuo, Govindarajulu Nagarajan, Shu-Mei Liang, Chi-Ming Liang, Chi-Min Chen
Rok vydání: 2007
Předmět:
CpG ODN
Cell Survival
Swine
CpG Oligodeoxynucleotide
[SDV.BC]Life Sciences [q-bio]/Cellular Biology
Biology
TLR9
03 medical and health sciences
proteomics
0302 clinical medicine
Heat shock protein
[SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB]
Animals
Enzyme Inhibitors
Cells
Cultured
Heat-Shock Proteins
030304 developmental biology
0303 health sciences
Dose-Response Relationship
Drug
General Veterinary
Gene Expression Profiling
[SDV.BA]Life Sciences [q-bio]/Animal biology
Spondin 1
apoptosis
Chloroquine
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Molecular biology
hemic and immune systems
Molecular biology
3. Good health
Hsp70
[SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
Gene Expression Regulation
Oligodeoxyribonucleotides
CpG site
Toll-Like Receptor 9
heat shock proteins
Leukocytes
Mononuclear
[SDV.IMM]Life Sciences [q-bio]/Immunology
Quercetin
[SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]
[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie
HSP60
Macrolides
Alpha chain
030215 immunology
Zdroj: Veterinary Research
Veterinary Research, BioMed Central, 2007, 38 (6), pp.795-808. ⟨10.1051/vetres:2007032⟩
ISSN: 1297-9716
0928-4249
DOI: 10.1051/vetres:2007032
Popis: International audience; The CpG motif within bacterial DNA is a potent immuno-stimulatory moiety. Here, using a 2-D electrophoretic approach, we investigated the effect of synthetic oligodeoxynucleotide containing a B type CpG motif (CpG-B ODN) on the protein expression profile of swine peripheral blood mononuclear cells (PBMC). We found that several proteins including spondin 1, N-acetolactate alpha linked acidic dipeptidase; V kappa light chain, T cell receptor variable alpha chain, heat shock protein (Hsp) 60, Hsp70, KIAA0857 protein, and PNAS-146 were up-regulated in PBMC by CpG-B ODN stimulation. Further studies showed that CpG-B ODN-mediated Hsp60, Hsp70 and Hsp90 expressions were closely associated with the TLR9 signalling pathway. Pretreatment with inhibitors of Hsp70, Hsp90 and TLR9 all blocked the CpG-B ODN-mediated anti-apoptotic effect in swine PBMC. These results suggest that CpG-B ODN treatment of swine PBMC may enhance the expression of biologically active proteins, notably spondin 1, V kappa light chain, T cell receptor variable alpha chain and Hsps, which may play an important role in CpG-B ODN-mediated activation of immune responses and enhancement of swine PBMC survival.
Databáze: OpenAIRE
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