CIP2A, an oncoprotein, is associated with cell proliferation, invasion and migration in laryngeal carcinoma cells
| Autor: | Yao-Wen Wang, Xudong Chen, Jian-Hua Zhang, Shi-Xiong Tang, Litao Zhang |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male Cancer Research Cell Apoptosis Biology Autoantigens 03 medical and health sciences 0302 clinical medicine Cell Movement medicine Biomarkers Tumor Tumor Cells Cultured Humans Neoplasm Invasiveness Phosphorylation Protein kinase B Laryngeal Neoplasms PI3K/AKT/mTOR pathway Cell Proliferation Oncogene Cell growth Intracellular Signaling Peptides and Proteins Membrane Proteins General Medicine Protein phosphatase 2 Cell cycle Middle Aged Prognosis Gene Expression Regulation Neoplastic Survival Rate 030104 developmental biology medicine.anatomical_structure Oncology 030220 oncology & carcinogenesis Cancer research Female A431 cells Follow-Up Studies |
| Zdroj: | Oncology reports. 38(2) |
| ISSN: | 1791-2431 |
| Popis: | Laryngeal carcinoma is one of the most common malignant tumors in otorhinolaryngology. Moreover, experimental investigation showed that cancerous inhibitor of protein phosphatase 2A (CIP2A) expressed highly in various cancers. Therefore, we investigated whether CIP2A can regulate the proliferation, invasion and migration by RNA interference in Hep-2 cells and AMC-NH-8 cells and further affect the activation of phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT) pathway. Overexpression of CIP2A was evaluated in tumor tissue and laryngeal cancer cell lines (Hep-2 and AMC-NH-8 cells) by real-time quantitative polymerase chain reaction (RT-qPCR) and western blot assay. In a follow-up experiment, we confirmed that CIP2A siRNA effectively suppressed the cell proliferation at 48 and 72 h, and arrested cell cycle at G0/G1 in Hep-2 cells and AMC-NH-8 cells. The invasion and migration of cell in siRNA CIP2A group were markedly inhibited. Moreover, the experimental results showed that the expression levels of invasion- and migration-related genes, including E-cadherin, metastasis-associated gene 1 (MTA1) and matrix metalloproteinases-2/9 (MMP-2/9), were regulated by CIP2A siRNA. Phosphorylation levels of PI3K and AKT proteins were reduced by CIP2A siRNA. Importantly, it suggested signaling through PI3K/Akt as a critical mechanism by which CIP2A siRNA may suppress cell proliferation, invasion and migration in laryngeal carcinoma cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|