Metabolic engineering of Serratia marcescens MG1 for enhanced production of (3R)-acetoin
| Autor: | Xin Lv, Liaoyuan Zhang, Yaling Shen, Lu Dai, Zhanqing Wang, Fangmin Bai |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Meso-2 030106 microbiology Biomedical Engineering Dehydrogenase Metabolic engineering 03 medical and health sciences chemistry.chemical_compound chemistry.chemical_classification biology Strain (chemistry) Renewable Energy Sustainability and the Environment Acetoin Research Glycerol dehydrogenase Serratia marcescens MG1 SlaR biology.organism_classification (3R)-AC 3-Butanediol dehydrogenase 030104 developmental biology Enzyme chemistry Biochemistry Transcriptional regulator Serratia marcescens Industrial and production engineering Food Science Biotechnology |
| Zdroj: | Bioresources and Bioprocessing |
| ISSN: | 2197-4365 |
| Popis: | Background Optically pure acetoin (AC) is an important platform chemical which has been widely used to synthesize novel optically active α-hydroxyketone derivatives and liquid crystal composites. Results In this study, slaC and gldA encoding meso-2,3-butanediol dehydrogenase (meso-2,3-BDH) and glycerol dehydrogenase (GDH), respectively, in S. marcescens MG1 were knocked out to block the conversion from AC to 2,3-butanediol (2,3-BD). The resulting strain MG14 was found to produce a large amount of optically pure (3R)-AC with a little 2,3-BD, indicating that another enzyme responsible for 2,3-BD formation except meso-2,3-BDH and GDH existed in the strain MG1. Furthermore, SlaR protein, a transcriptional activator of AC cluster, was overexpressed using P C promoter in the strain MG14, leading to enhancement of the (3R)-AC yield by 29.91%. The recombinant strain with overexpression of SlaR, designated as S. marcescens MG15, was used to perform medium optimization for improving (3R)-AC production. Conclusion Under the optimized conditions, 39.91 ± 1.35 g/l (3R)-AC was produced by strain MG15 with the productivity of 1.11 g/l h and the conversion rate of 80.13%. |
| Databáze: | OpenAIRE |
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