Autophagy activation is required for myofibroblast differentiation during healing of oral mucosa
| Autor: | Paola Pontecorvi, Simona Ceccarelli, Andrea Pilloni, Enrica Vescarelli, Cinzia Marchese, Francesco Dominici, Antonella Polimeni, Antonio Angeloni |
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| Rok vydání: | 2017 |
| Předmět: |
collagen
0301 basic medicine Adult Male Periodontal tissue Pathology medicine.medical_specialty periodontal tissues Biopsy Blotting Western Gingiva Fluorescent Antibody Technique Immunofluorescence Real-Time Polymerase Chain Reaction 03 medical and health sciences Western blot Autophagy Medicine Humans Oral mucosa Myofibroblasts Cells Cultured Wound Healing medicine.diagnostic_test business.industry autophagy gingiva myofibroblasts wound healing Mouth Mucosa Differential regulation Cell Differentiation Actins 030104 developmental biology medicine.anatomical_structure Tooth Extraction Periodontics Female Molar Third business Wound healing Myofibroblast |
| Zdroj: | Journal of clinical periodontology. 44(10) |
| ISSN: | 1600-051X |
| Popis: | Aim It is known that periodontal tissues heal faster that skin, and gingiva in particular heal without scar formation. The mechanisms regulating this behavior are still unclear. The aim of our work is to compare wound healing in oral mucosa and gingiva, investigating the role of αSMA-expressing myofibroblasts and autophagy. Materials and Methods Biopsies were obtained from 7 patients immediately before and 24h after vertical releasing incision in oral mucosa and attached gingiva. Both whole biopsies and primary cultures of fibroblasts derived from the same tissues were subjected to immunofluorescence, Western blot and qRT-PCR analyses. Results We demonstrated that in oral mucosa, characterized by partially fibrotic outcome during repair, the activation of autophagy determined an increase in αSMA and collagen 1a1 production. Conversely, wound healing did not stimulate autophagy in attached gingiva, and subsequently no increase in myofibroblast differentiation and collagen deposition could be seen, thus justifying its scareless outcome. Conclusion The elucidation of the differential regulation of autophagy in periodontal tissues and its correlation with myofibroblast differentiation and fibrotic outcome could allow the identification of new molecules involved in periodontal healing and the development of new surgical approaches for periodontal treatment that could improve the outcome of postoperative wounds. This article is protected by copyright. All rights reserved. |
| Databáze: | OpenAIRE |
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