Ex vivo penetration analysis and anti-inflammatory efficacy of the association of ferulic acid and UV filters
| Autor: | André Rolim Baby, Claudinéia Aparecida Sales de Oliveira Pinto, Maria Valéria Robles Velasco, Catarina Rosado, Rafael Sauce |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Coumaric Acids
Ultraviolet Rays Anti-Inflammatory Agents Pharmaceutical Science UV filter Human skin 02 engineering and technology 030226 pharmacology & pharmacy Ferulic acid 03 medical and health sciences chemistry.chemical_compound Ethylhexyl triazone 0302 clinical medicine In vivo Stratum corneum medicine Humans Skin Chromatography ANTIOXIDANTES Bemotrizinol 021001 nanoscience & nanotechnology medicine.anatomical_structure chemistry 0210 nano-technology Sunscreening Agents Ex vivo |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| Popis: | Background Unprotected chronic exposure to ultraviolet (UV) radiation generates many harmful effects to human skin and sunscreens are essential to health, however, traditional products do not provide enough protection against cutaneous oxidative stress, a process amplified by UV radiation. Therefore, the development of multifunctional photoprotective formulations seems to be a more efficacious approach, since these enable the absorption/reflection of UV radiation and maintain the cutaneous homeostasis. Objectives In the present study, ferulic acid (FA), a well-known antioxidant, has been combined with two UV filters, bemotrizinol and ethylhexyl triazone, and the safety and efficacy of this formulation has been assessed combining ex vivo and in vivo methods. Methods Skin permeation assays were performed by applying the formulation in the volar forearm of participants, after which consecutive samples of the stratum corneum were collected by tape stripping, and the quantification of FA, bemotrizinol and ethylhexyl triazone was performed by high-performance liquid chromatography (HPLC). Also, the FA anti-inflammatory action in combination with the UV filters was probed through a method employing Laser Doppler flowmetry to measure the vasodilatory response to methyl nicotinate topical application. Results Skin permeation assay was able to characterize the penetration depth of each substance. It should also be noted that a specific HPLC analytical method was developed in this study to enable the rapid simultaneous quantification of the three substances. Results from Laser Doppler flowmetry showed that the FA was able to mitigate the vasodilatory response. Conclusions FA proved to be a valuable resource in a multifunction sunscreen, not only providing an increase in the SPF of sunscreens, previously published, but also decreasing the extent of inflammation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|