A PCR-based Field Evaluation of Theileria Infections in Cattle and Ticks in Kenya a
Autor: | H. Kiara, D. Watt, O. A. E. Sparagano |
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Rok vydání: | 1998 |
Předmět: |
Veterinary medicine
medicine.medical_specialty Theileria parva Tick Polymerase Chain Reaction Sensitivity and Specificity General Biochemistry Genetics and Molecular Biology Ticks History and Philosophy of Science Theileria parasitic diseases Epidemiology Prevalence medicine Animals East Coast fever Parasite hosting DNA Primers Base Sequence Geography biology General Neuroscience Infection prevalence Central africa biology.organism_classification Kenya Virology Theileriasis Cattle |
Zdroj: | Annals of the New York Academy of Sciences. 849:69-77 |
ISSN: | 0077-8923 |
DOI: | 10.1111/j.1749-6632.1998.tb11035.x |
Popis: | Theileria parva is a hemoprotozoan parasite responsible for causing East Coast fever in east and central Africa. The vaccine currently available is an "infection and treatment" procedure which involves the injection of live sporozoites followed by drug therapy to prevent clinical illness. Before introducing potentially new strains of parasite into an area, however, it is crucial to check the disease situation in the field first. We looked at three different areas in Kenya: Limuru in which many cattle have already been vaccinated and Kitale and Kakamega which so far have not been vaccinated. Genus and species specific primers were used to test for the presence of Theileria species in blood and tick samples collected from the three areas. Limuru showed a cattle and tick infection prevalence of 27% and 2.3% respectively with T. parva. Kitale showed a cattle and tick infection prevalence of 100% and 14.2% respectively while Kakamega showed 100% and 0% respectively. Reasons for variations between areas involving vaccination status and epidemiological data are discussed. |
Databáze: | OpenAIRE |
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