Data for semi-permanent cationic coating for protein separations
| Autor: | C.A. Wilson, Lisa A. Holland, W.M. Penny, Cassandra L. Crihfield, Courtney J Kristoff, Lindsay M. Veltri |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
pH-stability
Protein adsorption engineering.material lcsh:Computer applications to medicine. Medical informatics Capillary electrophoresis 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Surface modification Coating Bromide Electroosmotic flow lcsh:Science (General) Semi-permanent coating 030304 developmental biology 0303 health sciences Reproducibility Multidisciplinary Chromatography Cationic polymerization Chemistry chemistry engineering lcsh:R858-859.7 Lysozyme 030217 neurology & neurosurgery lcsh:Q1-390 |
| Zdroj: | Data in Brief Data in Brief, Vol 29, Iss, Pp-(2020) |
| ISSN: | 2352-3409 |
| Popis: | Protein separations and analyses are fundamental to fields of study that include biochemistry, biology, physiology, drug discovery, pharmaceuticals, as well as agricultural and food based industries. Here, we provide the data from a novel phospholipid-cetyltrimethylammonium bromide coating capable of separating cationic and anionic proteins with high efficiency. Capillary electrophoresis separations of protein standards were utilized to characterize the performance of the novel coating. Using capillary electrophoresis with UV absorbance detection a working pH range of 4–9 was identified, with reproducibility in time ≤1% relative standard deviation, and plate counts for proteins as high as 480,000 plates (lysozyme, pH 7). Further details and results from these data are available in the work reported by Crihfield et al. and can be accessed at https://doi.org/10.1016/j.chroma.2019.460397 [1]. Keywords: Capillary electrophoresis, Semi-permanent coating, Surface modification, Protein adsorption, pH-stability, Electroosmotic flow |
| Databáze: | OpenAIRE |
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