Human monoclonal antibodies against West Nile virus from Japanese encephalitis-vaccinated volunteers
| Autor: | Atsushi Muraguchi, Eiji Konishi, Atsushi Yamanaka, Tatsuhiko Ozawa, Yoh Ohnuki, Hideyuki Masaki, Takahiro Yumisashi, Ikuko Aoyama, Tomohiko Takasaki, Hiroyuki Kishi |
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| Rok vydání: | 2018 |
| Předmět: |
Adult
0301 basic medicine Serotype medicine.drug_class viruses 030106 microbiology Cross Reactions Monoclonal antibody Virus 03 medical and health sciences Flaviviridae Neutralization Tests Virology medicine Animals Humans Encephalitis Japanese Neutralizing antibody Encephalitis Virus Japanese Pharmacology biology Japanese Encephalitis Vaccines Antibodies Monoclonal virus diseases Meningoencephalitis Japanese encephalitis biology.organism_classification medicine.disease Antibodies Neutralizing nervous system diseases Mice Inbred C57BL Flavivirus 030104 developmental biology Immunologic Techniques Leukocytes Mononuclear biology.protein Female West Nile virus West Nile Fever |
| Zdroj: | Antiviral Research. 154:58-65 |
| ISSN: | 0166-3542 |
| Popis: | West Nile virus (WNV) is a positive-sense single-stranded RNA flavivirus belonging to the Japanese encephalitis virus (JEV) serocomplex of the Flaviviridae family and causes mosquito-borne infections. Although most human infection cases are asymptomatic, approximately one in 150 infected individuals develops meningoencephalitis, with a mortality rate of 4-14%. While the development of human neutralizing antibody therapeutics against WNV is strongly anticipated, WNV is difficult to study in conventional laboratories due to its high safety level requirement. In this study, we established fully human WNV-neutralizing monoclonal antibodies from the peripheral blood mononuclear cells of inactivated-JEV-vaccinated individuals, and these antibodies exhibited WNV neutralization both in vitro and in vivo. Our results demonstrate a new antibody cross-reactivity strategy to develop immunological therapeutic reagents for WNV and other JEV serotype viruses. |
| Databáze: | OpenAIRE |
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