Quantification of granulocyte-macrophage colony-stimulating factor hypersensitivity in juvenile myelomonocytic leukemia by 3H-thymidine assay
| Autor: | Seiji Kojima, Nao Yoshida, Yin Yan Xu, Makito Tanaka, Nobuhiro Nishio, Yue Wang, Asahito Hama, Itzel Bustos Villalobos, Ayami Yoshimi, Hirokazu Hidaka, Yoshiyuki Takahashi, Hiroshi Yagasaki |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Male
Cancer Research Protein Tyrosine Phosphatase Non-Receptor Type 11 Biology Pathogenesis chemistry.chemical_compound medicine Humans Cell Proliferation Juvenile myelomonocytic leukemia Cell growth Granulocyte-Macrophage Colony-Stimulating Factor Infant Hematology medicine.disease PTPN11 Leukemia Granulocyte macrophage colony-stimulating factor Genes ras Oncology chemistry Ras Signaling Pathway Leukemia Myelomonocytic Juvenile Child Preschool Immunology Female Thymidine medicine.drug |
| Zdroj: | Leukemia research. 32(7) |
| ISSN: | 0145-2126 |
| Popis: | Recent molecular studies have revealed that the GM-CSF/RAS signaling pathway plays a central role in the pathogenesis of juvenile myelomonocytic leukemia (JMML). CFU-GM colony assay is an important test for GM-CSF hypersensitivity in patients with JMML, but requires specific skills. We established a simple and easy quantification method to test GM-CSF hypersensitivity, using a (3)H-thymidine assay. With this quantification method, JMML patients with RAS mutations showed significantly higher GM-CSF sensitivity than JMML patients with PTPN11 mutations. This method will be useful not only in the diagnosis of JMML, but also to evaluate the difference of GM-CSF sensitivity among patients. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect