Dengue Virus Type 3 Adaptive Changes during Epidemics in Sao Jose de Rio Preto, Brazil, 2006-2007

Autor: Christian Julian, Villabona-Arenas, Adriano, Mondini, Irene, Bosch, Diane J, Schimdt, Diane, Schimitt, Carlos E, Calzavara-Silva, Paolo M de A, Zanotto, Maurício L, Nogueira
Přispěvatelé: Universidade de São Paulo (USP), Universidade Estadual Paulista (Unesp), MIT, Tufts Univ, Fundacao Oswaldo Cruz Fiocruz, Harvard University--MIT Division of Health Sciences and Technology, Bosch, Irene
Jazyk: angličtina
Rok vydání: 2013
Předmět:
Zdroj: Web of Science
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
PLoS
Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
PLoS ONE, Vol 8, Iss 5, p e63496 (2013)
PLoS ONE
Popis: Made available in DSpace on 2014-12-03T13:08:43Z (GMT). No. of bitstreams: 0 Previous issue date: 2013-05-07Bitstream added on 2014-12-03T13:24:38Z : No. of bitstreams: 1 WOS000319654700151.pdf: 1120910 bytes, checksum: 67ccec12228a936946f09dd351ca1920 (MD5) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) INCT - Dengue Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Global dengue virus spread in tropical and sub-tropical regions has become a major international public health concern. It is evident that DENV genetic diversity plays a significant role in the immunopathology of the disease and that the identification of polymorphisms associated with adaptive responses is important for vaccine development. The investigation of naturally occurring genomic variants may play an important role in the comprehension of different adaptive strategies used by these mutants to evade the human immune system. In order to elucidate this role we sequenced the complete polyprotein-coding region of thirty-three DENV-3 isolates to characterize variants circulating under high endemicity in the city of Sao Jose de Rio Preto, Brazil, during the onset of the 2006-07 epidemic. By inferring the evolutionary history on a local-scale and estimating rates of synonymous (dS) and nonsynonimous (dN) substitutions, we have documented at least two different introductions of DENV-3 into the city and detected 10 polymorphic codon sites under significant positive selection (dN/dS > 1) and 8 under significant purifying selection (dN/dS < 1). We found several polymorphic amino acid coding sites in the envelope (15), NS1 (17), NS2A ( 11), and NS5 (24) genes, which suggests that these genes may be experiencing relatively recent adaptive changes. Furthermore, some polymorphisms correlated with changes in the immunogenicity of several epitopes. Our study highlights the existence of significant and informative DENV variability at the spatio-temporal scale of an urban outbreak. Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, LEMB, BR-05508 Sao Paulo, Brazil Univ Estadual Paulista Julio de Mesquita Filho Ar, Fac Ciencias Farmaceut, Dept Ciencias Biol, Lab Saude Publ, Sao Paulo, Brazil MIT, Div Hlth Sci & Technol, Cambridge, MA 02139 USA Tufts Univ, Dept Infect Dis & Global Hlth, Cummings Sch Vet Med, North Grafton, MA USA Fundacao Oswaldo Cruz Fiocruz, Ctr Pesquisas Rene Rachou CPqRR, LICM, Belo Horizonte, MG, Brazil Fac Med Sao Jose do Rio Preto, Lab Pesquisas Virol, Sao Jose Do Rio Preto, SP, Brazil Univ Estadual Paulista Julio de Mesquita Filho Ar, Fac Ciencias Farmaceut, Dept Ciencias Biol, Lab Saude Publ, Sao Paulo, Brazil FAPESP: 11/17071-2 FAPESP: 10/19059-7 FAPESP: 12/11733-6 FAPESP: 11/10458-9
Databáze: OpenAIRE
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