An asymmetric sheath controls flagellar supercoiling and motility in the leptospira spirochete
Autor: | Mathieu Picardeau, Ariel E. Mechaly, Elsio A. Wunder, Jun Liu, Fabiana San Martin, Albert I. Ko, Kimberley H. Gibson, Felipe Trajtenberg, Zhiguo Shang, Charles V. Sindelar, Megan R Brady, Alejandro Buschiazzo |
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Přispěvatelé: | Yale University School of Medicine, Institut Pasteur de Montevideo, Réseau International des Instituts Pasteur (RIIP), Yale School of Public Health (YSPH), Instituto Gonçalo Moniz / Gonçalo Moniz Research Centre - Fiocruz Bahia [Salvador, Brésil] (IGM), Fundação Oswaldo Cruz (FIOCRUZ), Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Department of Microbial Pathogenesis, Biologie des Spirochètes / Biology of Spirochetes, Institut Pasteur [Paris], Integrative Microbiology of Zoonotic Agents [Paris and Montevideo] (IMiZA), Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut Pasteur [Paris], Liste des financeurs :National Institutes of Health : Grants number R01 GM 110530 - U01 AI 088752 - R01 TW009504 - R01 AI052473 et R0 AI121207.Agencia Nacional de Investigacion e Innovacion : Grants number FCE_3_2016_1_126797 - ALI_1_2014_1_4982 et FCE_3_2016_1_126797.Agence Nationale de la Recherche : Grants number ANR-18-CE15-0027-1 et ANR-08-MIE-018.Pasteur International Joint Research Unit : Grant number Integrative Microbiology of Zoonotic Agents (IMiZA).Institut Pasteur, We gratefully acknowledge Garrett Debs for help with the RELION subtomogram averaging pre-processing step, including his customizations of published python scripts for Relion sub-tomogram averaging. We thank the IT Department from Institut Pasteur for protein modeling computations on the TARS cluster and the staff in the Yale High-Performance Computing facility for their maintenance of these facilities. We thank Prof. Felix Rey for providing insightful discussions about the manuscript., ANR-18-CE15-0027,LEPTOMOVE,Mécanismes moléculaires de la motilité chez les spirochètes: le modèle de l'endoflagelle des leptospires(2018), ANR-08-MIEN-0018,LEPTOVIR,Les leptospires : de la génétique à la pathogénèse(2008), Yale School of Medicine [New Haven, Connecticut] (YSM), Fundação Oswaldo Cruz / Oswaldo Cruz Foundation (FIOCRUZ), Institut Pasteur [Paris] (IP), Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut Pasteur [Paris] (IP) |
Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
Rotation
QH301-705.5 Science Movement Structural Biology and Molecular Biophysics infectious disease [SDV]Life Sciences [q-bio] Motility Flagellum General Biochemistry Genetics and Molecular Biology Protein filament 03 medical and health sciences leptospira Bacterial Proteins Leptospira molecular biophysics structural biology leptospirosis Biology (General) x-ray crystallography 030304 developmental biology 0303 health sciences Microbiology and Infectious Disease biology General Immunology and Microbiology 030306 microbiology Chemistry electron cryomicroscopy General Neuroscience 030302 biochemistry & molecular biology microbiology Periplasmic space General Medicine biology.organism_classification [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology cryo-tomography Flagella Biophysics DNA supercoil Medicine Other Bacteria Entire cell Research Article |
Zdroj: | eLife eLife, eLife Sciences Publication, 2020, 9, ⟨10.7554/eLife.53672⟩ eLife, Vol 9 (2020) eLife, 2020, 9, ⟨10.7554/eLife.53672⟩ |
ISSN: | 2050-084X |
DOI: | 10.7554/eLife.53672⟩ |
Popis: | Spirochete bacteria, including important pathogens, exhibit a distinctive means of swimming via undulations of the entire cell. Motility is powered by the rotation of supercoiled ‘endoflagella’ that wrap around the cell body, confined within the periplasmic space. To investigate the structural basis of flagellar supercoiling, which is critical for motility, we determined the structure of native flagellar filaments from the spirocheteLeptospiraby integrating high-resolution cryo-electron tomography and X-ray crystallography. We show that these filaments are coated by a highly asymmetric, multi-component sheath layer, contrasting with flagellin-only homopolymers previously observed in exoflagellated bacteria. Distinct sheath proteins localize to the filament inner and outer curvatures to define the supercoiling geometry, explaining a key functional attribute of the spirochete flagellum.One Sentence summaryThe corkscrew-like motility of Spirochete bacteria is enabled by a unique, asymmetrically constructed flagellum that wraps around the cell body within the periplasm. |
Databáze: | OpenAIRE |
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