An asymmetric sheath controls flagellar supercoiling and motility in the leptospira spirochete

Autor: Mathieu Picardeau, Ariel E. Mechaly, Elsio A. Wunder, Jun Liu, Fabiana San Martin, Albert I. Ko, Kimberley H. Gibson, Felipe Trajtenberg, Zhiguo Shang, Charles V. Sindelar, Megan R Brady, Alejandro Buschiazzo
Přispěvatelé: Yale University School of Medicine, Institut Pasteur de Montevideo, Réseau International des Instituts Pasteur (RIIP), Yale School of Public Health (YSPH), Instituto Gonçalo Moniz / Gonçalo Moniz Research Centre - Fiocruz Bahia [Salvador, Brésil] (IGM), Fundação Oswaldo Cruz (FIOCRUZ), Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Department of Microbial Pathogenesis, Biologie des Spirochètes / Biology of Spirochetes, Institut Pasteur [Paris], Integrative Microbiology of Zoonotic Agents [Paris and Montevideo] (IMiZA), Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut Pasteur [Paris], Liste des financeurs :National Institutes of Health : Grants number R01 GM 110530 - U01 AI 088752 - R01 TW009504 - R01 AI052473 et R0 AI121207.Agencia Nacional de Investigacion e Innovacion : Grants number FCE_3_2016_1_126797 - ALI_1_2014_1_4982 et FCE_3_2016_1_126797.Agence Nationale de la Recherche : Grants number ANR-18-CE15-0027-1 et ANR-08-MIE-018.Pasteur International Joint Research Unit : Grant number Integrative Microbiology of Zoonotic Agents (IMiZA).Institut Pasteur, We gratefully acknowledge Garrett Debs for help with the RELION subtomogram averaging pre-processing step, including his customizations of published python scripts for Relion sub-tomogram averaging. We thank the IT Department from Institut Pasteur for protein modeling computations on the TARS cluster and the staff in the Yale High-Performance Computing facility for their maintenance of these facilities. We thank Prof. Felix Rey for providing insightful discussions about the manuscript., ANR-18-CE15-0027,LEPTOMOVE,Mécanismes moléculaires de la motilité chez les spirochètes: le modèle de l'endoflagelle des leptospires(2018), ANR-08-MIEN-0018,LEPTOVIR,Les leptospires : de la génétique à la pathogénèse(2008), Yale School of Medicine [New Haven, Connecticut] (YSM), Fundação Oswaldo Cruz / Oswaldo Cruz Foundation (FIOCRUZ), Institut Pasteur [Paris] (IP), Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut Pasteur [Paris] (IP)
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Rotation
QH301-705.5
Science
Movement
Structural Biology and Molecular Biophysics
infectious disease
[SDV]Life Sciences [q-bio]
Motility
Flagellum
General Biochemistry
Genetics and Molecular Biology

Protein filament
03 medical and health sciences
leptospira
Bacterial Proteins
Leptospira
molecular biophysics
structural biology
leptospirosis
Biology (General)
x-ray crystallography
030304 developmental biology
0303 health sciences
Microbiology and Infectious Disease
biology
General Immunology and Microbiology
030306 microbiology
Chemistry
electron cryomicroscopy
General Neuroscience
030302 biochemistry & molecular biology
microbiology
Periplasmic space
General Medicine
biology.organism_classification
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
cryo-tomography
Flagella
Biophysics
DNA supercoil
Medicine
Other
Bacteria
Entire cell
Research Article
Zdroj: eLife
eLife, eLife Sciences Publication, 2020, 9, ⟨10.7554/eLife.53672⟩
eLife, Vol 9 (2020)
eLife, 2020, 9, ⟨10.7554/eLife.53672⟩
ISSN: 2050-084X
DOI: 10.7554/eLife.53672⟩
Popis: Spirochete bacteria, including important pathogens, exhibit a distinctive means of swimming via undulations of the entire cell. Motility is powered by the rotation of supercoiled ‘endoflagella’ that wrap around the cell body, confined within the periplasmic space. To investigate the structural basis of flagellar supercoiling, which is critical for motility, we determined the structure of native flagellar filaments from the spirocheteLeptospiraby integrating high-resolution cryo-electron tomography and X-ray crystallography. We show that these filaments are coated by a highly asymmetric, multi-component sheath layer, contrasting with flagellin-only homopolymers previously observed in exoflagellated bacteria. Distinct sheath proteins localize to the filament inner and outer curvatures to define the supercoiling geometry, explaining a key functional attribute of the spirochete flagellum.One Sentence summaryThe corkscrew-like motility of Spirochete bacteria is enabled by a unique, asymmetrically constructed flagellum that wraps around the cell body within the periplasm.
Databáze: OpenAIRE