Cytokine-induced Killer T Cells Enhance the Cytotoxicity Against Carboplatin-resistant Ovarian Cancer Cells
| Autor: | Ya Hsu Chiu, Jacqueline Whang-Peng, Liang Ming Lee, Der Yang Cho, Shao Chih Chiu, Chi Hao Hsiao, Yueh Pan, Ping Hsiao Shih, Yu Ching Wen |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Cancer Research
T cell Dose-Response Relationship Immunologic Antineoplastic Agents Immunotherapy Adoptive Carboplatin Interferon-gamma chemistry.chemical_compound Cytokine-Induced Killer Cells Interleukin-1alpha medicine Humans Cytotoxic T cell Cytotoxicity Cells Cultured Ovarian Neoplasms Cytokine-induced killer cell business.industry Drug Synergism General Medicine Natural killer T cell medicine.disease Combined Modality Therapy medicine.anatomical_structure Oncology chemistry Drug Resistance Neoplasm Cancer cell Leukocytes Mononuclear Cancer research Interleukin-2 Female Ovarian cancer business |
| Zdroj: | Anticancer Research. 40:3865-3872 |
| ISSN: | 1791-7530 0250-7005 |
| Popis: | Background/aim Ovarian cancer (OC) is typically diagnosed at an advanced stage with limitations for cure. Cytokine-induced killer (CIK) T cell therapy exerts significant cytotoxic effects against cancer cells and reduces the adverse effects of chemotherapy. Herein, we performed a flow cytometry-based method to evaluate the cytotoxicity of peripheral blood mononuclear cells-derived CIK cells against OC cells. Materials and methods The CIK cells were induced and expanded using an interferon-γ/IL-2-based xeno-free medium system. The cytotoxicity of CIK cells or carboplatin against OC cells was examined. Results The CIK cells showed an NK-like phenotypic characteristic and dose-dependently increased cytotoxicity against OC cells. We found that the number of advanced OC cells, which were more resistant to carboplatin, was dramatically decreased by an additional one-shot CIK treatment. Conclusion CIK cells have a potent cytotoxic ability that would be explored as an alternative strategy for cancer treatment in the near future. |
| Databáze: | OpenAIRE |
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