Comparison of several immunoassays used in drugs of abuse screening: Assessment against gold standard methods and calculation of measurement uncertainty
| Autor: | Ashraf Mina |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Pharmacology
Immunoassay Drugs of abuse Screening assessment Urine drug screening Chromatography medicine.diagnostic_test business.industry Uncertainty Gold standard (test) 030204 cardiovascular system & hematology Toxicology 030226 pharmacology & pharmacy EIA method Sensitivity and Specificity Substance Abuse Detection 03 medical and health sciences 0302 clinical medicine Pharmaceutical Preparations medicine Screening method Measurement uncertainty Humans business |
| Zdroj: | Journal of pharmacological and toxicological methods. 101 |
| ISSN: | 1873-488X |
| Popis: | Background Immunoassays provide simple, powerful and inexpensive screening methods for urine drug screening. Other substances and/or factors may interfere with the test and cause false or positive results. It is essential to understand the differences between methods to be able to evaluate their impact on the results. All evaluated immunoassays were assessed in comparison with GC–MS or LC-MS/MS, which are generally accepted as vigorous confirmation gold standard techniques. Methods CEDIA, DRI, EIA and EMIT II Plus screening immunoassays were evaluated on Beckman-Coulter AU5810 analyser. All results were confirmed using GC–MS or LC-MS/MS methods. Measurement Uncertainty for immunoassays was calculated by using standard deviation multiplied by 1.96 to cover 95% confidence interval of tested samples. Results No discrepancy was found between CEDIA and EMIT II Plus for cocaine, methadone, heroin, and benzodiazepines assays. No discrepancy was found between oxycodone DRI assay and Immunalysis enzyme immunoassay (EIA). Cannabinoids EMIT II Plus assay performed better than DRI assay. EMIT II Plus assays for amphetamine and ecstasy performed better than CEDIA amphetamine/ecstasy combined assay. Opiates EMIT II Plus assay performed better than CEDIA assay. Fentanyl Ark EIA method performed slightly better than the DRI method. Buprenorphine CEDIA second-generation assay performed better than CEDIA first-generation assay, EMIT II Plus assay and EMIT II Plus with added Beta-Glucuronidase assay. Measurement Uncertainty for immunoassays was calculated and tabulated. Conclusions This study covered a fundamental gap in available knowledge by evaluating the performance of screening the current new generation of immunoassays methods for drugs of abuse in urine against gold standard methods. |
| Databáze: | OpenAIRE |
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