The Hfq-like protein NrfA of the phototrophic purple bacteriumRhodobacter capsulatuscontrols nitrogen fixation via regulation ofnifAandanfAexpression
| Autor: | Meike Gendrullis, Bernd Masepohl, Werner Klipp, Daphne Giaourakis, Karsten Raabe, Thomas Drepper |
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| Rok vydání: | 2002 |
| Předmět: |
Molecular Sequence Data
medicine.disease_cause Microbiology Rhodobacter capsulatus Bacterial Proteins Nitrogen Fixation Nitrogenase Genetics medicine Amino Acid Sequence Molecular Biology Rhodospirillaceae Escherichia coli Regulator gene Molybdenum Rhodobacter Bacteria biology RNA-Binding Proteins Gene Expression Regulation Bacterial biology.organism_classification Culture Media DNA-Binding Proteins Biochemistry Azorhizobium caulinodans Trans-Activators Nitrogen fixation bacteria Diazotroph Transcription Factors |
| Zdroj: | FEMS Microbiology Letters. 215:221-227 |
| ISSN: | 1574-6968 0378-1097 |
| DOI: | 10.1111/j.1574-6968.2002.tb11394.x |
| Popis: | The Rhodobacter capsulatus nrfA gene product exhibits extensive similarity to the nif (nitrogen fixation) regulatory factor NrfA of Azorhizobium caulinodans and the nucleoid-associated protein Hfq of Escherichia coli. Mutational analysis revealed that, in contrast to the situation in A. caulinodans, NrfA is not essential for diazotrophic growth of R. capsulatus, but it is required for maximal growth rates with N(2) as sole nitrogen source via either molybdenum nitrogenase or the alternative nitrogenase. NrfA was shown to control N(2) fixation in R. capsulatus at the level of expression of the regulatory genes nifA1, nifA2 and anfA, encoding the transcriptional activators of all the other nitrogen fixation genes. |
| Databáze: | OpenAIRE |
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