Comparative 16S rRNA Analysis of Lake Bacterioplankton Reveals Globally Distributed Phylogenetic Clusters Including an Abundant Group of Actinobacteria
| Autor: | Evgeny Zaichikov, Natalia V. Belkova, Rudolf Amann, Jakob Pernthaler, Annelie Pernthaler, Frank Oliver Glöckner, Ludmilla Denissova |
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| Rok vydání: | 2000 |
| Předmět: |
DNA
Bacterial Molecular Sequence Data Fresh Water DNA Ribosomal Applied Microbiology and Biotechnology Freshwater ecosystem Microbial Ecology Actinobacteria Abundance (ecology) RNA Ribosomal 16S Proteobacteria Animals Bacteroides Cloning Molecular Ecosystem In Situ Hybridization Fluorescence Phylogeny Biomass (ecology) Ecology biology Bacteroidetes Sequence Analysis DNA Bacterioplankton Plankton biology.organism_classification Limnohabitans Food Science Biotechnology Polynucleobacter |
| Zdroj: | Applied and Environmental Microbiology. 66:5053-5065 |
| ISSN: | 1098-5336 0099-2240 |
| Popis: | In a search for cosmopolitan phylogenetic clusters of freshwater bacteria, we recovered a total of 190 full and partial 16S ribosomal DNA (rDNA) sequences from three different lakes (Lake Gossenköllesee, Austria; Lake Fuchskuhle, Germany; and Lake Baikal, Russia). The phylogenetic comparison with the currently available rDNA data set showed that our sequences fall into 16 clusters, which otherwise include bacterial rDNA sequences of primarily freshwater and soil, but not marine, origin. Six of the clusters were affiliated with the α, four were affiliated with the β, and one was affiliated with the γ subclass of the Proteobacteria ; four were affiliated with the Cytophaga-Flavobacterium-Bacteroides group; and one was affiliated with the class Actinobacteria (formerly known as the high-G+C gram-positive bacteria). The latter cluster (hgcI) is monophyletic and so far includes only sequences directly retrieved from aquatic environments. Fluorescence in situ hybridization (FISH) with probes specific for the hgcI cluster showed abundances of up to 1.7 × 10 5 cells ml −1 in Lake Gossenköllesee, with strong seasonal fluctuations, and high abundances in the two other lakes investigated. Cell size measurements revealed that Actinobacteria in Lake Gossenköllesee can account for up to 63% of the bacterioplankton biomass. A combination of phylogenetic analysis and FISH was used to reveal 16 globally distributed sequence clusters and to confirm the broad distribution, abundance, and high biomass of members of the class Actinobacteria in freshwater ecosystems. |
| Databáze: | OpenAIRE |
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