Conducting Polymer Based Enzyme Electrodes Fabricated by Invertase and Polyphenol Oxidase
| Autor: | Elif Doğan, Ayşe Elif Böyükbayram |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Cultural Studies
Conductive polymer Linguistics and Language History Immobilized enzyme Chemistry Chemistry Multidisciplinary Carbon Fiber Electrode Conducting Polymer Enzyme Immobilization Polyphenol Oxidase Polyphenol oxidase Language and Linguistics Invertase Chemical engineering Anthropology Carbon fiber electrode Enzyme Electrodes Kimya Ortak Disiplinler |
| Zdroj: | Volume: 4, Issue: 2 113-119 Kocaeli Journal of Science and Engineering |
| ISSN: | 2667-484X |
| Popis: | Novel carbon fiber enzyme electrodes were constructed and evaluated. Fabrication of electrodes was performed via electrochemical deposition of a conducting matrice composed of polythiophene and polypyrrole (PTh–PPy) onto carbon fiber substrates. The enzyme was entrapped into the matrix during electropolymerization. Resultant biosensors represented higher kinetic parameters, Vmax and Km, in comparison to PPy matrice, which are 2.471 ± 0.150 mol min1- electrode1- and 30.60 ± 5.30 mM for invertase, 0.056 ± 0.012 mol min1- electrode1- and 842.00 ± 37.50 mM for polyphenol oxidase respectively. Optimum pH and temperature of the immobilized enzyme within PTh–PPy composite indicates that this matrice provides a more protective environment. The detection limit (LOD) of polyphenols was obtained as 0.037 mg mL1-. Polyphenol oxidase enzyme electrodes were proved to be used for the determination of polyphenolic substances in real samples and the results were confirmed by the Folin-Ciocalteau method |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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