Long non-coding RNA MAGI2-as3 is a new player with a tumor suppressive role in high grade serous ovarian carcinoma
| Autor: | Priyanka Gokulnath, Mariastella Zannini, Ichcha Manipur, Tiziana de Cristofaro, Amata Amy Soriano, Mario Rosario Guarracino, Tina Di Palma |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Cancer Research
Biology medicine.disease_cause Article RNA-sequencing analysis Transcriptome 03 medical and health sciences 0302 clinical medicine Epithelial Ovarian Cancer Fallopian Tube High Grade Serous Ovarian Cancer competing endogenous RNA lncRNA MAGI2-AS3 microRNA non-coding RNA tumor suppressor Ovarian carcinoma medicine Non-coding RNA 030304 developmental biology 0303 health sciences Competing endogenous RNA MicroRNA Tumor suppressor Long non-coding RNA 3. Good health Serous fluid LncRNA MAGI2-AS3 Oncology 030220 oncology & carcinogenesis Cancer research Carcinogenesis |
| Zdroj: | Cancers (basel) Cancers Volume 11 Issue 12 Cancers (Basel) 11 (2019). doi:10.3390/cancers11122008 info:cnr-pdr/source/autori:Gokulnath P.; de Cristofaro T.; Manipur I.; Di Palma T.; Soriano A.A.; Guarracino M.R.; Zannini M./titolo:Long non-coding RNA MAGI2-as3 is a new player with a tumor suppressive role in high grade serous ovarian carcinoma/doi:10.3390%2Fcancers11122008/rivista:Cancers (Basel)/anno:2019/pagina_da:/pagina_a:/intervallo_pagine:/volume:11 |
| DOI: | 10.3390/cancers11122008 |
| Popis: | High-Grade Serous Ovarian Carcinoma (HGSC) is the most incidental and lethal subtype of epithelial ovarian cancer (EOC) with a high mortality rate of nearly 65%. Recent findings aimed at understanding the pathogenesis of HGSC have attributed its principal source as the Fallopian Tube (FT). To further comprehend the exact mechanism of carcinogenesis, which is still less known, we performed a transcriptome analysis comparing FT and HGSC. Our study aims at exploring new players involved in the development of HGSC from FT, along with their signaling network, and we chose to focus on non-coding RNAs. Non-coding RNAs (ncRNAs) are increasingly observed to be the major regulators of several cellular processes and could have key functions as biological markers, as well as even a therapeutic approach. The most physiologically relevant and significantly dysregulated non-coding RNAs were identified bioinformatically. After analyzing the trend in HGSC and other cancers, MAGI2-AS3 was observed to be an important player in EOC. We assessed its tumor-suppressive role in EOC by means of various assays. Further, we mapped its signaling pathway using its role as a miRNA sponge to predict the miRNAs binding to MAGI2AS3 and showed it experimentally. We conclude that MAGI2-AS3 acts as a tumor suppressor in EOC, specifically in HGSC by sponging miR-15-5p, miR-374a-5p and miR-374b-5p, and altering downstream signaling of certain mRNAs through a ceRNA network. |
| Databáze: | OpenAIRE |
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