Five BoLA-DRB3 genotypes detected in Egyptian buffalo infected with Foot and Mouth disease virus serotype O
| Autor: | Hussein A. Hussein, Ayman H. El-Deeb, Othman E. Othman, Muhammad G. Khodary |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Serotype lcsh:QH426-470 Sequence analysis lcsh:Biotechnology animal diseases Egyptian buffalo FMDV 03 medical and health sciences lcsh:TP248.13-248.65 parasitic diseases Genotype medicine General Materials Science biology Foot-and-mouth disease 0402 animal and dairy science RNA virus 04 agricultural and veterinary sciences biology.organism_classification medicine.disease 040201 dairy & animal science Virology Animal Biotechnology lcsh:Genetics 030104 developmental biology Genetic marker Qt-PCR BoLA-DRB3 RFLP Restriction fragment length polymorphism Foot-and-mouth disease virus |
| Zdroj: | Journal of Genetic Engineering & Biotechnology Journal of Genetic Engineering and Biotechnology, Vol 16, Iss 2, Pp 513-518 (2018) |
| ISSN: | 1687-157X |
| Popis: | Foot and Mouth disease (FMD) is a contagious disease leads to economically loss in livestock production all over the world. This serious disease is caused due to the infection of the animal with a single-stranded RNA virus (FMDV). This study aimed to investigate the genetic polymorphism of BoLA-DRB3 gene in Egyptian buffalo as a candidate genetic marker included in multi-factorial process of FMD resistance/susceptibility. Also this work aimed to genetically characterization and serotyping of circulating FMD virus in Egypt during 2016.For serotyping of FMDV, RT-PCR was used for FMDV-positive samples and the results declared the presence of serotype O in all tested animals. The sequence analysis of FMDV samples revealed five different patterns for the detected serotype O which were submitted to GenBank under the accession Nos.: MG017361–MG017365.The 302-bp amplified fragments from BoLA-DRB3 exon 2 were digested with HaeIII endonuclease and the results showed that the presence of five BoLA-DRB3 genotypes, among them the genotype AA might be associated with FMD-resistance (P |
| Databáze: | OpenAIRE |
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