Growth on poly(l-lactic acid) porous scaffold preserves CD73 and CD90 immunophenotype markers of rat bone marrow mesenchymal stromal cells
| Autor: | Anna Tampieri, Elena Bassi, Giulia Spaletta, Michele Iafisco, Roberto Giardino, Nicoletta Zini, Luca Cattini, Monica Sandri, Fulvio Barbaro, Roberto Toni, Milena Fini, S. Mosca, Simone Sprio, A. Zamparelli, Annapaola Parrilli, D. Dallatana, Nadir M. Maraldi |
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| Přispěvatelé: | Alessandra Zamparelli, Nicoletta Zini, Luca Cattini, Giulia Spaletta, Davide Dallatana, Elena Bassi, Fulvio Barbaro, Michele Iafisco, Salvatore Mosca, Annapaola Parrilli, Milena Fini, Roberto Giardino, Monica Sandri, Simone Sprio, Anna Tampieri, Nadir M. Maraldi, Roberto Toni |
| Rok vydání: | 2014 |
| Předmět: |
Male
Materials science Polymers Polyesters Biomedical Engineering Biophysics Motility Bone Marrow Cells Bioengineering biomateria Immunophenotyping Rats Sprague-Dawley Biomaterials Tissue engineering Materials Testing Animals CD90 Lactic Acid Intermediate filament 5'-Nucleotidase Cells Cultured Cell Proliferation Tissue Scaffolds Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells Adhesion In vitro Rats Cell biology Numerical modelling Thy-1 Antigens Desmin REGENERATIVE MEDICINE Porosity Biomarkers Biomedical engineering |
| Zdroj: | Journal of materials science. Materials in medicine 25 (2014): 2421–2436. doi:10.1007/s10856-014-5259-4 info:cnr-pdr/source/autori:Zamparelli A, Zini N, Cattini L, Spaletta G, Dallatana D, Bassi E, Barbaro F, Iafisco M, Mosca S, Parrilli A, Fini M, Giardino R, Sandri M, Sprio S, Tampieri A, Maraldi NM, Toni R./titolo:Growth on poly(L-lactic acid) porous scaffold preserves CD73 and CD90 immunophenotype markers of rat bone marrow mesenchymal stromal cells/doi:10.1007%2Fs10856-014-5259-4/rivista:Journal of materials science. Materials in medicine/anno:2014/pagina_da:2421/pagina_a:2436/intervallo_pagine:2421–2436/volume:25 |
| ISSN: | 1573-4838 0957-4530 |
| Popis: | Few data are available on the effect of biomaterials on surface antigens of mammalian bone marrow-derived, adult mesenchymal stromal cells (MSCs). Since poly(L-lactic acid) or PLLA is largely used in tissue engineering of human bones, and we are developing a reverse engineering program to prototype with biomaterials the vascular architecture of bones for their bioartificial reconstruction, both in humans and animal models, we have studied the effect of porous, flat and smooth PLLA scaffolds on the immunophenotype of in vitro grown, rat MSCs in the absence of any coating, co-polymeric enrichment, and differentiation stimuli. Similar to controls on plastic, we show that our PLLA scaffold does not modify the distribution of some surface markers in rat MSCs. In particular, the maintained expression of CD73 and CD90 on two different sub-populations (small and large cells) is consistent with their adhesion to the PLLA scaffold through specialized appendages, and to their prominent content in actin. In addition, our PLLA scaffold favours retention of the intermediate filament desmin, believed a putative marker of undifferentiated state. Finally, it preserves all rat MSCs morphotypes, and allows for their survival, adhesion to the substrate, and replication. Remarkably, a subpopulation of rat MSCs grown on our PLLA scaffold exhibited formation of membrane protrusions of uncertain significance, although in a size range and morphology compatible with either motility blebs or shedding vesicles. In summary, our PLLA scaffold has no detrimental effect on a number of features of rat MSCs, primarily the expression of CD73 and CD90. |
| Databáze: | OpenAIRE |
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