Multi-center evaluation of one commercial and 12 in-house real-time PCR assays for detection of Mycoplasma pneumoniae
| Autor: | Darja Keše, Randi Føns Petersen, Alvaro J. Benitez, Theo Hoogenboezem, Jolanda Maaskant, Wendy W. J. Unger, Ran Nir-Paz, Meike Rosenblatt, Karolina Gullsby, Annemarie M. C. van Rossum, Cécile Bébéar, Jonas M. Winchell, Birgit Henrich, Roger Dumke, Søren A. Uldum, Katherine Loens, Sabine Pereyre, Suzan D. Pas, Allon E. Moses, Carlos Hidalgo-Grass, Ayelet Michael-Gayego, D. Ursi, Victoria J. Chalker |
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| Přispěvatelé: | Immunology, Virology, Pediatrics, EACMID Study Grp Mycoplasma Infect |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Microbiology (medical) DNA Bacterial Mycoplasma pneumoniae 030106 microbiology General Medicine Biology medicine.disease_cause Real-Time Polymerase Chain Reaction Virology 03 medical and health sciences Infectious Diseases Real-time polymerase chain reaction Pneumonia Mycoplasma medicine Humans Human medicine Genome Bacterial |
| Zdroj: | Diagnostic Microbiology & Infectious Disease, 88(2), 111-114. Elsevier Inc. Diagnostic microbiology and infectious disease |
| ISSN: | 1879-0070 0732-8893 |
| Popis: | Detection of Mycoplasma pneumoniae by real-time PCR is not yet standardized across laboratories. We have implemented a standardization protocol to compare the performance of thirteen commercial and in-house approaches. Despite differences on threshold values of samples, all assays were able to detect at least 20 M. pneumoniae genomes per reaction. (C) 2017 Elsevier Inc. All rights reserved. |
| Databáze: | OpenAIRE |
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