Fabrication and evaluation of microgrooved polymers as peripheral nerve conduits
| Autor: | Hsiao-Chiang Ni, Shan-hui Hsu, Po Seng Lu, Chien-Hsiang Su |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Male
Silicon Materials science Time Factors Polymers Polyesters Cell Biomedical Engineering Nerve guidance conduit Cell Culture Techniques Gene Expression Biocompatible Materials Rats Sprague-Dawley Neurotrophic factors Nerve Growth Factor medicine Cell Adhesion Animals Dimethylpolysiloxanes Lactic Acid Peripheral Nerves Cell adhesion Fluorescent Antibody Technique Indirect Molecular Biology Cells Cultured Cytoskeleton Chitosan biology Regeneration (biology) Brain-Derived Neurotrophic Factor S100 Proteins Glioma Sciatic Nerve Actins Vinculin Rats medicine.anatomical_structure Biodegradation Environmental nervous system Animals Newborn Cell culture biology.protein Feasibility Studies Sciatic nerve Schwann Cells Neurotrophin Biomedical engineering |
| Zdroj: | Biomedical microdevices. 9(5) |
| ISSN: | 1387-2176 |
| Popis: | Cell alignment plays an important role in the repair of damaged peripheral nerves. The aligned Schwann cells could direct the axonal outgrowth during nerve reconstruction. One way of aligning Schwann cells is to use surface grooves in micrometric dimensions. In this study, microgrooves on chitosan or poly(D,L-lactide) (PLA) were fabricated and the behaviors of Schwann cells and glial cell line C6 on these surfaces were examined. It was found that Schwann cells and C6 cells could be successfully aligned by the microgrooves, and express the genes related to the production of neurotrophic factors. The polymer conduits with microgrooves on the inner surface were implanted in rats to repair the damaged sciatic nerve. The microgrooved conduits were demonstrated to enhance peripheral nerve regeneration as compared to the smooth conduits. |
| Databáze: | OpenAIRE |
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