Human Cerebral Organoids and Fetal Brain Tissue Share Proteomic Similarities
| Autor: | Juliana Minardi Nascimento, Verônica M. Saia-Cereda, Rafaela C. Sartore, Rodrigo Madeiro da Costa, Clarissa S. Schitine, Hercules Rezende Freitas, Michael Murgu, Ricardo A. de Melo Reis, Stevens K. Rehen, Daniel Martins-de-Souza |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
DIVERSITY Synaptogenesis Biology 03 medical and health sciences Cell and Developmental Biology 0302 clinical medicine proteomics stem cells medicine Organoid Progenitor cell Induced pluripotent stem cell lcsh:QH301-705.5 PRECURSOR NEURONS Original Research Science & Technology COMPLEX brain organoids neural cells Neurogenesis DEFECTS Cell Biology Human brain oligodendrocyte progenitors MODEL DIFFERENTIATION 030104 developmental biology medicine.anatomical_structure lcsh:Biology (General) 030220 oncology & carcinogenesis Axon guidance Stem cell Life Sciences & Biomedicine PLURIPOTENT STEM-CELLS Neuroscience SYSTEM Developmental Biology |
| Zdroj: | Frontiers in Cell and Developmental Biology Frontiers in Cell and Developmental Biology, Vol 7 (2019) |
| ISSN: | 2296-634X |
| Popis: | The limited access to functional human brain tissue has led to the development of stem cell-based alternative models. The differentiation of human pluripotent stem cells into cerebral organoids with self-organized architecture has created novel opportunities to study the early stages of the human cerebral formation. Here we applied state-of-the-art label-free shotgun proteomics to compare the proteome of stem cell-derived cerebral organoids to the human fetal brain. We identified 3,073 proteins associated with different developmental stages, from neural progenitors to neurons, astrocytes, or oligodendrocytes. The major protein groups are associated with neurogenesis, axon guidance, synaptogenesis, and cortical brain development. Glial cell proteins related to cell growth and maintenance, energy metabolism, cell communication, and signaling were also described. Our data support the variety of cells and neural network functional pathways observed within cell-derived cerebral organoids, confirming their usefulness as an alternative model. The characterization of brain organoid proteome is key to explore, in a dish, atypical and disrupted processes during brain development or neurodevelopmental, neurodegenerative, and neuropsychiatric diseases. ispartof: FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY vol:7 ispartof: location:Switzerland status: published |
| Databáze: | OpenAIRE |
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