Identification of a novel oxygenase capable of regiospecific hydroxylation of D-limonene into (+)-trans-carveol
| Autor: | H. L. van Beek, Wouter A Duetz, Marco W. Fraaije, M. Groeneveld |
|---|---|
| Přispěvatelé: | Enzymology, Biotechnology |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cumene Carveol EXPRESSION Oxygenase TOLUENE DIOXYGENASE Stereochemistry 030106 microbiology METABOLISM D-Limone Hydroxylation Biochemistry ISOPROPYLBENZENE 03 medical and health sciences chemistry.chemical_compound Biotransformation Drug Discovery MONOOXYGENATION CRYSTAL-STRUCTURE Indole test biology Organic Chemistry biology.organism_classification Carvone Pseudomonas putida COMPONENT PSEUDOMONAS-PUTIDA 030104 developmental biology chemistry Heterologous expression BIOTRANSFORMATION PLASMID |
| Zdroj: | Tetrahedron, 72(46), 7263-7267. PERGAMON-ELSEVIER SCIENCE LTD |
| ISSN: | 1464-5416 0040-4020 |
| DOI: | 10.1016/j.tet.2015.12.061 |
| Popis: | By genome sequencing and analysis, we have been able to identify a gene cluster encoding a four component oxygenase which is able to oxidize D-limonene, L-limonene, cumene, and indole. Heterologous expression of the complete oxygenase system in Pseudomonas putida S12 allowed efficient and highly regiospecific biotransformation of D-limonene into (+)-trans-carveol. The discovered oxygenase represents a promising biocatalyst for the biotechnological valorisation of D-limonene. (C) 2015 Elsevier Ltd. All rights reserved. |
| Databáze: | OpenAIRE |
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