Effects of gamma rays on rat testis tissue according to the morphological parameters and immunohistochemistry: radioprotective role of silymarin
| Autor: | Reza Ahadi, Mohsen Marzban, Daryoush Fatehi, Hossien Masoumi, Maryam Anjamshoa, Parnia Jafari |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
lcsh:R5-920 Pathology medicine.medical_specialty TUNEL assay Leydig cell Spermatid Radiotherapy Radioprotector Lumen (anatomy) Biology Epithelium Andrology 03 medical and health sciences 030104 developmental biology medicine.anatomical_structure Apoptosis medicine Immunohistochemistry Original Article Antioxidant lcsh:Medicine (General) Spermatogenesis Silymarin |
| Zdroj: | Electronic Physician Electronic Physician, Vol 9, Iss 6, Pp 4524-4532 (2017) |
| ISSN: | 2008-5842 |
| Popis: | OBJECTIVE To determine the radioprotective effects of Silymarin in adult male Sprague-Dawley rats irradiated with γ-rays. METHODS The present experimental study was performed in Tehran University of Medical Sciences, Tehran, Iran from December 2009 to March 2010. The study was performed on 40 rats, which were randomly and equally divided into four groups: 1) control group: neither received Silymarin nor irradiated with γ-rays; 2) γ-irradiation group: testis region exposed to 2Gy of γ-rays; 3) Silymarin & γ-irradiation: rats received 100 mg/kg of Silymarin 24hrs before exposure to 2Gy of γ-rays; 4) Silymarin & γ-irradiation: rats received 200 mg/kg of Silymarin 24hrs before exposure to 2Gy of γ-rays. After animal experiments and preparing the tissue sections, different histological and histomorphological parameters of seminiferous tubules and the biological characteristics of Leydig cells were evaluated applying quantitative assessment, Johnson scoring, and Leydig cell apoptosis assay by TUNEL method. The data were analyzed applying ANOVA and Tukey's post hoc test, using SPSS software (V.19). RESULTS Irradiation of 2 Gy γ-rays to the testis of the rats significantly affected the frequency of spermatogonia, primary spermatocyte, round spermatid, spermatozoa, seminiferous tube and lumen diameters, thickness of the epithelium, Leydig cell nuclear diameter and volume, epithelium height, and apoptotic cells (p |
| Databáze: | OpenAIRE |
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