Animal protein-free OptiXcell and shortened equilibration periods can replace egg yolk-based extender and slow cooling for rhinoceros semen cryopreservation
| Autor: | Terri L. Roth, Jessye Wojtusik, Monica A. Stoops |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Male
food.ingredient Semen Rhinoceros General Biochemistry Genetics and Molecular Biology Cryopreservation law.invention 03 medical and health sciences Cryoprotective Agents 0302 clinical medicine Animal science food law Yolk Animals Horses 030219 obstetrics & reproductive medicine biology Chemistry Ceratotherium simum Cell Membrane Extender 0402 animal and dairy science 04 agricultural and veterinary sciences General Medicine biology.organism_classification Egg Yolk Spermatozoa 040201 dairy & animal science Sperm Semen cryopreservation Cold Temperature Semen Analysis Sperm Motility General Agricultural and Biological Sciences Semen Preservation |
| Zdroj: | Cryobiology. 89:21-25 |
| ISSN: | 0011-2240 |
| DOI: | 10.1016/j.cryobiol.2019.06.003 |
| Popis: | OptiXcell (OP) was tested as an animal protein-free alternative to an egg yolk-based extender for rhinoceros semen cryopreservation and shorter chilling/equilibration periods were evaluated. Semen was collected from three rhinoceros species: black (Diceros bicornis; n = 2), white (Ceratotherium simum; n = 2), and greater one-horned (GOH; Rhinoceros unicornis; n = 3). Controls were diluted with equine extender (EQ) or OP and equilibrated for 1 h. Treatments were diluted with extender and cooled for 15 min (fast: FEQ; FOP) or not cooled (immediate: IEQ; IOP), prior to cryopreservation. Motility decreased post-thaw (EQ: 50.7 ± 5.2%; OP: 52.9 ± 3.4%) from fresh (82.9 ± 2.9%), was higher in OP than IOP (38.6 ± 4.9%; P ≤ 0.05) and decreased over time (P ≤ 0.05). Post-thaw acrosomal integrity was lower in EQ, FEQ, and IEQ (56.9 ± 0.7; 56.6 ± 4.5; 54.9 ± 2.9%) than OP, FOP, IOP (71.8 ± 4.7; 71.9 ± 3.8; 69.9 ± 4.5%) and fresh (72.6 ± 1.4%; P ≤ 0.05). Progression and viability were lower in EQ (2.8 ± 0.2; 61.9 ± 7.4%) and OP (3.1 ± 0.2; 53.4 ± 6.9%) than fresh (3.7 ± 0.2; 87.2 ± 1.3%), decreased over time (P ≤ 0.05) but not different among treatments (P > 0.05). Morphology did not differ between fresh (75.0 ± 4.9% normal) and any treatment group (70.0–77.8%) or over time (P > 0.05). OptiXcell is comparable to egg yolk-based EQ when used for rhinoceros semen cryopreservation. Furthermore, chilling/equilibration can be reduced with little impact on sperm characteristics. |
| Databáze: | OpenAIRE |
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