A submerged duckweed mutant with abundant starch accumulation for bioethanol production
| Autor: | Gongke Zhou, Yubin Ma, Shumin Wang, Yu Wang, Yingzhen Kong, Guo He, Yu Liu, Xianfeng Tang, Hua Xu, Changjiang Yu |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
submergence
Renewable Energy Sustainability and the Environment Chemistry Starch duckweed starch lcsh:TJ807-830 Mutant lcsh:Renewable energy sources food and beverages Forestry lcsh:HD9502-9502.5 Heavy ion irradiation lcsh:Energy industries. Energy policy. Fuel trade sub‐1 mutant chemistry.chemical_compound Biofuel heavy‐ion irradiation Food science Waste Management and Disposal Agronomy and Crop Science bioethanol |
| Zdroj: | GCB Bioenergy, Vol 12, Iss 12, Pp 1078-1091 (2020) |
| ISSN: | 1757-1707 1757-1693 |
| Popis: | Duckweed is one kind of promising bioenergy plant with prominent advantages such as fast growth rate and high starch content. However, almost all previous studies focused on the natural duckweed germplasms. In this study, heavy‐ion irradiation was used to establish a mutant library of Lemna aequinoctialis 6002, and one mutant named submarine‐1 (sub‐1) was screened, which could accumulate more starch but with smaller granules. Unexpectedly, under proper external growth conditions such as poor nutritional status and insufficient growth space, sub‐1 mutant would sink underwater due to formation of dense tissue structure and large amount of fine starch particles with the extension of cultivation time. The starch content in the sinking sub‐1 increased to over 45% (dry weight) and was 12% higher than the floating sub‐1, highlighting that submergence can be considered as a spontaneous and efficient indicator for screening of high‐starch duckweed. Additionally, the saccharification efficiency of starch and ethanol yield had increased in sub‐1 mutant compared to the wild type. Based on the unique characteristics of sub‐1 mutant, a cultivation model of submerged duckweed in a simulated aquaculture pond was designed to get more starch‐rich biomass, enabling effective production of renewable bioenergy. |
| Databáze: | OpenAIRE |
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