Protection by Polaprezinc Against Radiation-Induced Apoptosis in Rat Jejunal Crypt Cells
| Autor: | Kumio Okaichi, Masahiro Nakashima, Kazuko Shichijo, Mutsumi Matsuu-Matsuyama, Ichiro Sekine, Takashi Uemura, Daisuke Niino, Toshiyuki Nakayama |
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| Rok vydání: | 2008 |
| Předmět: |
Male
p53 Health Toxicology and Mutagenesis Crypt Apoptosis Radiation-Protective Agents Polaprezinc Biology Haematoxylin Radiation Dosage Radiation-induced apoptosis Jejunum chemistry.chemical_compound Western blot Organometallic Compounds medicine Animals Radiology Nuclear Medicine and imaging Rats Wistar Cells Cultured Radiation TUNEL assay Dose-Response Relationship Drug Eosin medicine.diagnostic_test Carnosine Dose-Response Relationship Radiation Molecular biology Rats Intestine medicine.anatomical_structure chemistry Biochemistry Zinc Compounds |
| Zdroj: | Journal of Radiation Research. 49:341-347 |
| ISSN: | 1349-9157 0449-3060 |
| Popis: | Polaprezinc, an anti-ulcer drug, is a chelate compound consisting of zinc and L-carnosine. Polaprezinc has been shown to prevent gastric mucosal injury. The anti ulcer effects of polaprezinc have been ascribed to its antioxidative property. The effect of polaprezinc on ionizing radiation-induced apoptosis was studied in the jejunal epithelial crypt cells of rats. Seven-to eight week-old Wistar rats, which were treated with 100 mg/kg of polaprezinc orally 1h before irradiation or 2% carboxymethyl cellulose sodium in controls, were exposed to whole body X-ray irradiation at 2 Gy. The number of apoptotic cells per jejunum crypt was counted in haematoxylin and eosin stained sections at 0-6 h after irradiation. TUNEL positive cells and immunopositive cells for active caspase-3 per crypt were also counted. Accumulation of p53, p21^ and Bax expression in the jejunum after irradiation were examined by Western blot analyses. Polaprezinc treatment given prior to radiation resulted in a significant reduction in numbers of apoptotic cells, TUNEL positive cells and active caspase-3 immunopositive cells in jejunal crypt cells. Polaprezinc treatment resulted in decreases of p53 accumulation, p21^ and Bax expression after irradiation. Polaprezinc has a protective effect against ionizing radiation induced apoptosis in rat jejunal crypt cells. Journal of Radiation Research, 49(4), pp.341-347; 2008 |
| Databáze: | OpenAIRE |
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