| Popis: |
Trigonella foenum-graecum L. (fenugreek) leaves are nutritionally rich green leafy vegetables consumed in South-East Asian and Middle-Eastern countries. Scientific data suggests properties including anti-inflammatory, antipyretic, anti-nociception, and hepatoprotective. However, the molecules responsible for these properties are yet to be identified. In the present study, we have isolated and characterized an anti-inflammatory nutraceutical molecule based on bioassay from the fresh leaves of fenugreek. The lipoxygenase inhibition assay revealed 79.9 �� 1.1% and 69.0 �� 2.9% inhibition in ethanolic and aqueous extracts, respectively. The ethanolic extract purified on a semi-prep HPLC column yielded a nutraceutical molecule quercetin glycoside (QG) having potent inhibition of lipoxygenase (75.3 �� 1.6%) at 10 mM and hyaluronidase (67.4 �� 4.0%) at 2 mM, respectively. The QG structure was elucidated by spectroscopic data, including FTIR, UHPLC-MS/MS, and 1D and 2D NMR as Quercetin-3-O-diglucoside-7-O-glucoside. QG also suppressed ROS, NO, and IL-6 production in LPS stimulated RAW264.7 cells. Radical scavenging assays of DPPH and ABTS revealed the potent antioxidant activity of QG with an EC50 of 245.5 and 68.8 ��M, respectively. Our results demonstrate that newly characterized quercetin glycoside from fresh leaves of fenugreek possesses potential anti-inflammatory and antioxidant activities. |
