N-Methyl-d-aspartate receptor and L-type voltage-gated Ca2+ channel activation mediate proline-rich tyrosine kinase 2 phosphorylation during cerebral ischemia in rats
| Autor: | Jun Guo, Bo Song, Xinzhen Fu, Guang-Yi Zhang, Fanjie Meng, Xue-bo Yan |
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| Rok vydání: | 2004 |
| Předmět: |
Male
medicine.medical_specialty Calcium Channels L-Type Receptors N-Methyl-D-Aspartate Brain Ischemia Rats Sprague-Dawley Ca2+/calmodulin-dependent protein kinase Internal medicine medicine Animals Enzyme Inhibitors Phosphorylation Protein kinase C Voltage-dependent calcium channel Chemistry General Neuroscience Calcium channel Protein-Tyrosine Kinases Calcium Channel Blockers Rats Cell biology Focal Adhesion Kinase 2 Endocrinology NMDA receptor Signal transduction Proto-oncogene tyrosine-protein kinase Src |
| Zdroj: | Neuroscience Letters. 355:177-180 |
| ISSN: | 0304-3940 |
| DOI: | 10.1016/j.neulet.2003.10.076 |
| Popis: | Cerebral ischemia induces rapid efflux of glutamate into the extracellular space contributing to excessive activation of glutamate receptors in postsynaptic cells, particularly N-methyl-D-aspartate (NMDA) receptors, which triggers the neuron lesion through calcium overload. Our studies indicated that cerebral ischemia stimulated the rapid activation of nonreceptor tyrosine kinases proline-rich tyrosine kinase 2 (Pyk2) and Src and the binding to Pyk2 activated the latter. Pyk2 activation significantly depends on the increase of the intracellular calcium level; blockage of both calcium ion channel NMDA receptors and L-type voltage-gated Ca2+ channel (L-VGCC), respectively, could effectively inhibit phosphorylation of Pyk2 in early ischemia episodes. Moreover, pretreatment with the protein kinase C inhibitor (chelerythrine chloride) reduced the ischemia-induced activation of Pyk2. Noticeably, CaMKII, a family of calcium/calmodulin-dependent kinases, also may be involved in the regulation of Pyk2 activity because its inhibitor KN62 attenuated Pyk2 phosphorylation during ischemia. Together with previous studies, these results indicate that calcium influx elicited by active NMDA receptors and L-VGCC triggers the Pyk2-Src signaling pathway mediated by PKC, which aggravates cerebral ischemia lesions through up-regulating the function of NMDA receptors after the onset of ischemia, and also could be regulated partly by CaM-dependent kinases like CaMKII. |
| Databáze: | OpenAIRE |
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