Serum influence onin-vitrogene delivery using microbubble-assisted ultrasound

Autor:	Jean-Michel Escoffre, Patrick Vourc'h, Aya Zeghimi, Rose-Anne Thépault, Ayache Bouakaz, Anthony Novell
Přispěvatelé:	Escoffre, Jean-Michel, Imagerie et cerveau (iBrain - Inserm U1253 - UNIV Tours ), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Tours (UT), Université de Tours-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Tours (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Rok vydání:	2014
Předmět:	[SDV.BIO]Life Sciences [q-bio]/Biotechnology Cell Survival Genetic enhancement Pharmaceutical Science Gene delivery Pharmacology Permeability Cell Line Tumor Humans Ultrasonics Viability assay ComputingMilieux_MISCELLANEOUS Microbubbles Chemistry business.industry Osmolar Concentration Ultrasound Gene Transfer Techniques DNA Genetic Therapy Transfection Hydrogen-Ion Concentration [SDV.SP]Life Sciences [q-bio]/Pharmaceutical sciences Molecular biology In vitro [SDV.BIO] Life Sciences [q-bio]/Biotechnology Bioavailability [SDV.SP] Life Sciences [q-bio]/Pharmaceutical sciences Glioblastoma business Sonoporation Plasmids
Zdroj:	Journal of Drug Targeting Journal of Drug Targeting, Informa Healthcare, 2014, 22 (8), pp.748-760. ⟨10.3109/1061186X.2014.921922⟩
ISSN:	1029-2330 1061-186X
DOI:	10.3109/1061186x.2014.921922
Popis:	Plasmid DNA (pDNA) is attractive molecule for gene therapy. pDNA-targeted delivery by efficient and safe methods is required to enhance its intra-tissue bioavailability. Among non-viral methods, sonoporation has become a promising method for in-vitro and in-vivo pDNA delivery. The efficiency of non-viral delivery methods of pDNA is generally limited by the presence of serum.The aim of this study was to evaluate the influence of serum on in-vitro pDNA delivery using microbubble-assisted ultrasound.The effects of a range of serum concentrations (0-50%) on efficiency of in-vitro pDNA delivery by sonoporation were determined on human glioblastoma cells. Furthermore, the influence of the serum on cell viability, membrane permeabilization, microbubble destruction, and pDNA topology were also assessed.In-vitro results showed that a low serum concentration (i.e. ≤1%) induced a significant increase in transfection level through an increase in cell viability. However, a high serum concentration (i.e. ≥5%) resulted in a significant decrease in cell transfection, which was not associated with a decrease in membrane permeabilization or loss in cell viability. This decrease in transfection level was in fact positively correlated to changes in pDNA topology.Serum influences the efficiency of in-vitro pDNA delivery by sonoporation through change in pDNA topology.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6aaf765f06644cc5c22a041a3c34f2fa https://doi.org/10.3109/1061186x.2014.921922 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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