Identification of TTP mRNA targets in human dendritic cells reveals TTP as a critical regulator of dendritic cell maturation
| Autor: | Seth A. Brooks, W.H. Davin Townley-Tilson, Kristen M. Deleault, Jillian Emmons, Robert H. Gross, Stephen J. Skinner, Michael L. Whitfield |
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| Rok vydání: | 2008 |
| Předmět: |
Chemokine
Cellular differentiation Genes MHC Class II Molecular Sequence Data Genes MHC Class I Ligands Polymerase Chain Reaction Article Chemokine receptor Tristetraprolin Genes Reporter Sequence Homology Nucleic Acid MHC class I Humans Indoleamine-Pyrrole 2 3 -Dioxygenase RNA Messenger Molecular Biology 3' Untranslated Regions Oligonucleotide Array Sequence Analysis Regulation of gene expression MHC class II biology Base Sequence Cell Differentiation Dendritic cell DNA Dendritic Cells Acquired immune system Molecular biology Cell biology Gene Expression Regulation biology.protein Receptors Chemokine B7-2 Antigen Chemokines |
| Zdroj: | RNA (New York, N.Y.). 14(5) |
| ISSN: | 1469-9001 |
| Popis: | Dendritic cells provide a critical link between innate and adaptive immunity and are essential to prime a naive T-cell response. The transition from immature dendritic cells to mature dendritic cells involves numerous changes in gene expression; however, the role of post-transcriptional changes in this process has been largely ignored. Tristetraprolin is an AU-rich element mRNA-binding protein that has been shown to regulate the stability of a number of cytokines and chemokines of mRNAs. Using TTP immunoprecipitations and Affymetrix GeneChips, we identified 393 messages as putative TTP mRNA targets in human dendritic cells. Gene ontology analysis revealed that ∼25% of the identified mRNAs are associated with protein synthesis. We also identified six MHC Class I alleles, five MHC Class II alleles, seven chemokine and chemokine receptor genes, indoleamine 2,3 dioxygenase, and CD86 as putative TTP ligands. Real-time PCR was used to validate the GeneChip data for 15 putative target genes and functional studies performed for six target genes. These data establish that TTP regulates the expression of DUSP1, IDO, SOD2, CD86, and MHC Class I-B and F via the 3′-untranslated region of each gene. A novel finding is the demonstration that TTP can interact with and regulate the expression of non-AU-rich element-containing messages. The data implicate TTP as having a broader role in regulating and limiting the immune response than previously suspected. |
| Databáze: | OpenAIRE |
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