Qualitative Differences in the Behavior of Pneumococcal Deoxyribonucleic Acids Transforming to the Same Capsular Type
| Autor: | Harriet P. Bernheimer, Robert Austrian, Ingbritt E. Wermundsen |
|---|---|
| Rok vydání: | 1967 |
| Předmět: |
DNA
Bacterial chemistry.chemical_classification Genetics Mutation Polysaccharides Bacterial Chromosome Mapping Genetics and Molecular Biology Biology Polysaccharide medicine.disease_cause Microbiology Genome chemistry.chemical_compound Transformation (genetics) Streptococcus pneumoniae Biochemistry chemistry Streptomycin medicine Molecular Biology Capsular type Gene DNA |
| Zdroj: | Journal of Bacteriology. 93:320-333 |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.93.1.320-333.1967 |
| Popis: | A method is described for estimating quantitatively the frequency of transformation of pneumococci to new capsular types. It is found that, when S- III cells are exposed to deoxyribonucleic acid (DNA) from wild-type I strains, transformation to SI occurs at a frequency 20 to 60 times that of transformation to the binary type SI-III. SI markers on DNA isolated from binary strains behave qualitatively and quantitatively in a different manner from the same markers on DNA from wild-type I strains and will transform S- III cells only to SI-III. Strains are described which produce only one capsular polysaccharide, but which are genetically binary and carry a second capsular genome with a mutated gene so that the second polysaccharide is not produced. Stability and other characteristics of binary strains are discussed, and one hypothesis for the genetic organization of binary strains is presented. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|