Control and signal processing by transcriptional interference

Autor: Prasuna Ratna, Attila Becskei, Janos Z. Kelemen, Antoine Buetti-Dinh, Rosemarie Ungricht, Chetak Shetty
Přispěvatelé: University of Zurich, Becskei, A
Jazyk: angličtina
Rok vydání: 2009
Předmět:
Transcriptional Activation
Transcription
Genetic
Saccharomyces cerevisiae
Gene Expression
Genetics and Molecular Biology
1100 General Agricultural and Biological Sciences
Biology
Binding
Competitive
General Biochemistry
Genetics and Molecular Biology
noncoding transcription
2604 Applied Mathematics
1300 General Biochemistry
Genetics and Molecular Biology
Transcription (biology)
2400 General Immunology and Microbiology
Report
Gene Expression Regulation
Fungal
Gene expression
Promoter Regions
Genetic
Psychological repression
Gene
Polymerase
Genetics
promoter
General Immunology and Microbiology
Models
Genetic
Activator (genetics)
Applied Mathematics
Systems Biology
DNA
biology.organism_classification
Flow Cytometry
10124 Institute of Molecular Life Sciences
Cell biology
Protein Structure
Tertiary
Kinetics
Computational Theory and Mathematics
General Biochemistry
biology.protein
570 Life sciences
biology
U7 Systems Biology / Functional Genomics
Signal transduction
Genome
Fungal
General Agricultural and Biological Sciences
repression
Information Systems
Signal Transduction
Zdroj: Molecular Systems Biology
ISSN: 1744-4292
Popis: A transcriptional activator can suppress gene expression by interfering with transcription initiated by another activator. Transcriptional interference has been increasingly recognized as a regulatory mechanism of gene expression. The signals received by the two antagonistically acting activators are combined by the polymerase trafficking along the DNA. We have designed a dual-control genetic system in yeast to explore this antagonism systematically. Antagonism by an upstream activator bears the hallmarks of competitive inhibition, whereas a downstream activator inhibits gene expression non-competitively. When gene expression is induced weakly, the antagonistic activator can have a positive effect and can even trigger paradoxical activation. Equilibrium and non-equilibrium models of transcription shed light on the mechanism by which interference converts signals, and reveals that self-antagonism of activators imitates the behavior of feed-forward loops. Indeed, a synthetic circuit generates a bell-shaped response, so that the induction of expression is limited to a narrow range of the input signal. The identification of conserved regulatory principles of interference will help to predict the transcriptional response of genes in their genomic context.
Databáze: OpenAIRE
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