Bacteriophage-Based Biosensing of
| Autor: | Thais J. Oliveira, Waldemar Bonventi Junior, Marta M. D. C. Vila, Victor M. Balcão, Liliam K. Harada, Matthieu Tubino, Fernanda C. Moreli, Erica C. Silva, José Martins de Oliveira Junior |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
medicine.drug_class
Alginates lcsh:Biotechnology Clinical Biochemistry Antibiotics Virulence Biosensing Techniques medicine.disease_cause Article Microbiology Bacteriophage 03 medical and health sciences bacterial biosensing lcsh:TP248.13-248.65 Drug Resistance Multiple Bacterial medicine Humans Bacteriophages Pathogen 030304 developmental biology 0303 health sciences biology 030306 microbiology Pseudomonas aeruginosa bio-reactive polymeric matrix Hydrogels General Medicine biology.organism_classification chromogenic/bioluminescent bio-hydrogel Lytic cycle immobilization and structural/functional stabilization Bacterial virus bacteriophage particles Bacteria |
| Zdroj: | Biosensors, Vol 11, Iss 124, p 124 (2021) Biosensors Volume 11 Issue 4 |
| ISSN: | 2079-6374 |
| Popis: | During the last decennium, it has become widely accepted that ubiquitous bacterial viruses, or bacteriophages, exert enormous influences on our planet’s biosphere, killing between 4–50% of the daily produced bacteria and constituting the largest genetic diversity pool on our planet. Currently, bacterial infections linked to healthcare services are widespread, which, when associated with the increasing surge of antibiotic-resistant microorganisms, play a major role in patient morbidity and mortality. In this scenario, Pseudomonas aeruginosa alone is responsible for ca. 13–15% of all hospital-acquired infections. The pathogen P. aeruginosa is an opportunistic one, being endowed with metabolic versatility and high (both intrinsic and acquired) resistance to antibiotics. Bacteriophages (or phages) have been recognized as a tool with high potential for the detection of bacterial infections since these metabolically inert entities specifically attach to, and lyse, bacterial host cells, thus, allowing confirmation of the presence of viable cells. In the research effort described herein, three different phages with broad lytic spectrum capable of infecting P. aeruginosa were isolated from environmental sources. The isolated phages were elected on the basis of their ability to form clear and distinctive plaques, which is a hallmark characteristic of virulent phages. Next, their structural and functional stabilization was achieved via entrapment within the matrix of porous alginate, biopolymeric, and bio-reactive, chromogenic hydrogels aiming at their use as sensitive matrices producing both color changes and/or light emissions evolving from a reaction with (released) cytoplasmic moieties, as a bio-detection kit for P. aeruginosa cells. Full physicochemical and biological characterization of the isolated bacteriophages was the subject of a previous research paper. |
| Databáze: | OpenAIRE |
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