Evaluation of a Rapid IgG4 Lateral Flow Dipstick Test to Detect Strongyloides stercoralis Infection in Northeast Thailand
| Autor: | Jiraporn Sithithaworn, Nor Mustaiqazah Juri, Chanika Worasith, Nor Suhada Anuar, Kulthida Kopolrat, Rahmah Noordin, Phattharaphon Wongphutorn, Paiboon Sithithaworn, Sirowan Ruantip |
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| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
Antibodies Helminth Diagnostic tools Sensitivity and Specificity Gastroenterology Strongyloides stercoralis Feces Virology Internal medicine Animals Humans Medicine Serologic Tests Igg elisa biology business.industry Tropical disease Articles Helminth Proteins Dipstick Thailand biology.organism_classification medicine.disease Recombinant Proteins Infectious Diseases Immunoglobulin G Strongyloidiasis Parasitology business |
| Zdroj: | Am J Trop Med Hyg |
| ISSN: | 1476-1645 0002-9637 |
| Popis: | Strongyloides stercoralis affects more than half a billion people worldwide, and hyperinfection in immunocompromised patients can be fatal. Elimination of this neglected tropical disease requires field-applicable diagnostic tools. We conducted a laboratory evaluation of a lateral flow rapid dipstick test (SsRapid™) using sera samples from a Strongyloides-endemic area in northeast Thailand. Group 1 was S. stercoralis-positive and larvae- and/or antibody-positive (according to the IgG ELISA) (N = 100). Group 2 had negative fecal examination and IgG ELISA results (N = 25). Group 3 had other parasitic infections and negative IgG ELISA results (N = 25). The results showed good diagnostic sensitivity (82%) and excellent specificity (96%). Suggested improvements in the SsRapid™ test include increased diagnostic sensitivity and conversion to the more robust cassette format. Field studies should be performed as well. |
| Databáze: | OpenAIRE |
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