Pneumococcal colonisation is an asymptomatic event in healthy adults using an experimental human colonisation model

Autor: Carole A. Hancock, Daniela M. Ferreira, Stephen B. Gordon, Daniella McLenaghan, Angela D. Wright, Victoria Connor, Duolao Wang, Ryan E. Robinson, Ashleigh Trimble, Andrea M. Collins
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Serotype
Male
RNA viruses
Pulmonology
medicine.medical_treatment
Respiratory System
medicine.disease_cause
Pathology and Laboratory Medicine
Pediatrics
Nasopharynx
qv_771
Medicine and Health Sciences
Respiratory system
Saline
Multidisciplinary
wc_217
Pneumococcus
Middle Aged
Healthy Volunteers
Bacterial Pathogens
Streptococcus pneumoniae
Medical Microbiology
Viral Pathogens
Viruses
Medicine
Female
medicine.symptom
Symptom Assessment
Pathogens
Anatomy
Pediatric Infections
Research Article
Adult
medicine.medical_specialty
Nasal Provocation Tests
Science
030106 microbiology
Nose
Serogroup
Asymptomatic
Microbiology
Virus
Pneumococcal Infections
03 medical and health sciences
Internal medicine
Virology
medicine
Humans
Adults
Microbial Pathogens
Bacteria
business.industry
Organisms
Biology and Life Sciences
Streptococcus
Pneumonia
Pneumococcal
medicine.disease
Colonisation
Pneumonia
Nasal Mucosa
030104 developmental biology
Age Groups
People and Places
Paramyxoviruses
Respiratory Infections
Pharynx
Population Groupings
Respiratory Syncytial Virus
business
qw_142
Digestive System
Viral Transmission and Infection
Zdroj: PLoS ONE, Vol 15, Iss 3, p e0229558 (2020)
PLoS ONE
ISSN: 1932-6203
Popis: Introduction\ud Pneumococcal colonisation is regarded as a pre-requisite for developing pneumococcal disease. In children previous studies have reported pneumococcal colonisation to be a symptomatic event and described a relationship between symptom severity/frequency and colonisation density. The evidence for this in adults is lacking in the literature. This study uses the experimental human pneumococcal challenge (EHPC) model to explore whether pneumococcal colonisation is a symptomatic event in healthy adults.\ud \ud Methods\ud Healthy participants aged 18–50 were recruited and inoculated intra-nasally with either Streptococcus pneumoniae (serotypes 6B, 23F) or saline as a control. Respiratory viral swabs were obtained prior to inoculation. Nasal and non-nasal symptoms were then assessed using a modified Likert score between 1 (no symptoms) to 7 (cannot function). The rate of symptoms reported between the two groups was compared and a correlation analysis performed.\ud \ud Results\ud Data from 54 participants were analysed. 46 were inoculated with S. pneumoniae (29 with serotype 6B, 17 with serotype 23F) and 8 received saline (control). In total, 14 became experimentally colonised (30.4%), all of which were inoculated with serotype 6B. There was no statistically significant difference in nasal (p = 0.45) or non-nasal symptoms (p = 0.28) between the inoculation group and the control group. In those who were colonised there was no direct correlation between colonisation density and symptom severity. In the 22% (12/52) who were co-colonised, with pneumococcus and respiratory viruses, there was no statistical difference in either nasal or non-nasal symptoms (virus positive p = 0.74 and virus negative p = 1.0).\ud \ud Conclusion\ud Pneumococcal colonisation using the EHPC model is asymptomatic in healthy adults, regardless of pneumococcal density or viral co-colonisation.
Databáze: OpenAIRE
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