Modeling and Visualizing Bacterial Colony Purification Without the Use of Bacteria or Laboratory Equipment †
Autor: | Grace L. Axler-DiPerte |
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Jazyk: | angličtina |
Rok vydání: | 2017 |
Předmět: |
QH301-705.5
education Paintbrush Streak Tips & Tools Biology General Biochemistry Genetics and Molecular Biology pure culture technique Education quadrant streak 03 medical and health sciences 0302 clinical medicine Mixed culture 030212 general & internal medicine Biology (General) lcsh:QH301-705.5 Simulation lcsh:LC8-6691 microbiology laboratory skills LC8-6691 General Immunology and Microbiology lcsh:Special aspects of education 05 social sciences 050301 education Inoculation loop Special aspects of education lcsh:Biology (General) Biochemical engineering General Agricultural and Biological Sciences bacteriology 0503 education Bacterial colony |
Zdroj: | Journal of Microbiology & Biology Education Journal of Microbiology & Biology Education, Vol 18, Iss 2 (2017) |
ISSN: | 1935-7885 1935-7877 |
Popis: | Microorganisms typically exist in diverse and heterogeneous communities within their various environmental niches. The isolation of an individual species from these communities is an essential laboratory skill to study of the properties and behaviors of that organism. To achieve this separation, the “quadrant streak” for single colony purification is often included in undergraduate microbiology laboratory curricula. To aid student mastery of this technique, I have developed a simulated culture purification activity that allows students to immediately visualize the dilution and separation of an artificial microbial community with the goal of isolating purified colonies. This tool uses readily available, inexpensive, art supplies to simulate a mixed bacterial culture. The “mixed culture” consists of craft glitter of at least two distinct colors, held together with water-soluble, highly pigmented watercolor or gouache paint. Students practice aseptic technique by using a paintbrush to mimic an inoculating loop to streak and dilute the culture on a piece of cardstock. Sterilization of the “loop” is simulated by rinsing the brush. Students will immediately self-assess whether they are correctly performing the quadrant streak, rather than having to wait until the next laboratory session for bacteria to grow, which may allow them to master the technique sooner. |
Databáze: | OpenAIRE |
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