Stability of retrovirally transduced markers in a rat cell line
| Autor: | N. A. Dower, Louis Siminovitch, James C. Stone |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Hypoxanthine Phosphoribosyltransferase
Genes Viral Genetic Vectors Biology Transfection Thymidine Kinase Cell Line Viral vector Plasmid Transduction Genetic Gene expression Genetics Animals Simplexvirus Gene Genetic transfer Cell Biology General Medicine Provirus Molecular biology Rats Genes Hypoxanthine-guanine phosphoribosyltransferase Thymidine kinase Moloney murine leukemia virus Plasmids |
| Zdroj: | Somatic Cell and Molecular Genetics. 12:575-583 |
| ISSN: | 1572-9931 0740-7750 |
| DOI: | 10.1007/bf01671943 |
| Popis: | A MoMLV-based retroviral vector capable of transmitting and expressing both the human hypoxanthine phosphoribosyltransferase (hprt) coding sequence and the Herpes simplex type 1 thymidine kinase (tk) gene has been constructed. After infection of a rat cell line, cell clones were selected on the basis of expressing both markers. They were subsequently found to contain a single provirus of the expected topology. The ease with which loss of expression of the markers can be monitored has allowed us to make observations on the stability of proviral genes. In particular, we have found indirect evidence of strong position effects on proviral gene expression by comparing the characteristic frequency of marker loss in different clonal proviral lines. Effects of the selection protocol on the apparent frequency of variants have also been noted. Finally, a combination of molecular and genetic observations lead us to invoke chromosome loss as the major factor influencing marker stability in this system. |
| Databáze: | OpenAIRE |
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