Evaluation of three different genomic regions for detection of bovine leukemia virus by real-time PCR
| Autor: | Antônio Augusto Fonseca Júnior, Mateus Laguardia Nascimento, Marcelo Fernandes Camargos, Bruna Lopes Bueno, Jenner Karlisson Pimenta dos Reis, Luciana Rabello Ferreira, Anselmo Vasconcelos Rivetti Júnior |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
Bovine leukemia virus viruses Veterinary Microbiology - Research Paper Bovine leukosis Reproducibility of Results Genomics Biology Enzootic Bovine Leukosis biology.organism_classification Real-Time Polymerase Chain Reaction Microbiology Virology Real-time polymerase chain reaction Medical microbiology Media Technology medicine Leukemia Virus Bovine Animals Cattle Genus Deltaretrovirus |
| Zdroj: | Braz J Microbiol |
| Popis: | Bovine leukemia virus (BLV) is an oncogenic member of the genus Deltaretrovirus. BLV infects cattle worldwide and is responsible for significant economic losses. The objective of this study was to validate real-time quantitative PCR (qPCR) for the detection of BLV. After identification of the most efficient qPCR, the limits of detection, repeatability, and reproducibility were determined. The results indicate that qPCR can be easily reproduced between laboratories with high sensitivity. The test variation was low in samples from lesions suggestive of bovine leukosis or whole blood. |
| Databáze: | OpenAIRE |
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