Intranasal immunisation with Stx2B-Tir-Stx1B-Zot protein leads to decreased shedding in goats after challenge with Escherichia coli O157:H7
| Autor: | Wen Lb, Wang Xm, He Kw, Ye Q, Luan Xt, Ni Yx, Li B, Yu Zy, Guo Rl, Zhou Jm, Mao Ah, Zhang Xh, Zhao Pd |
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| Rok vydání: | 2012 |
| Předmět: |
Male
Biology Escherichia coli O157 Shiga Toxin 1 medicine.disease_cause Shiga Toxin 2 Microbiology law.invention Excretion Random Allocation law medicine Animals Humans Escherichia coli Administration Intranasal Escherichia coli Infections Feces Bacterial Shedding Goat Diseases General Veterinary Escherichia coli Vaccines Inoculation Escherichia coli Proteins Goats STX1B General Medicine Virology Recombinant DNA biology.protein Nasal administration Antibody |
| Zdroj: | Veterinary Record. 170:178-178 |
| ISSN: | 0042-4900 |
| DOI: | 10.1136/vr.100325 |
| Popis: | Ruminants are an important reservoir of Escherichia coli O157:H7. To reduce E coli O157:H7 excretion by these animals could play a key role in prevention and control of human infections. In the present study, the authors used 12 three-month-old goats to evaluate the efficacy of intranasal administration of the Stx2B-Tir-Stx1B-Zot protein. These goats were inoculated on days 0 and 21 and infected with 10 10 colony-forming units (cfu) of E coli O157:H7 by oral inoculation on day 36. Faecal shedding was monitored daily for two weeks. All of six goats immunised with recombinant protein elicited significant Stx2b-Tir-Stx1b-Zot-specific serum IgG antibodies, and three of them also showed production of antigen-specific IgA in faeces. The immunised goats showed much less shedding of E coli O157:H7 after challenge. These results demonstrate the potential for the use of Stx2B-Tir-Stx1B-Zot protein in mucosal vaccine formulations to prevent colonisation and shedding of E coli O157:H7 in goats. |
| Databáze: | OpenAIRE |
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