In vitro biocompatibility of nanostructured endodontic materials using SCAP cells
| Autor: | Bojana Ćetenović, Dejan Markovic, Vukoman Jokanović, Božana Čolović, Snežana Pašalić, Sasa Vasilijic |
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| Rok vydání: | 2017 |
| Předmět: |
Biocompatibility
Strategy and Management 02 engineering and technology 03 medical and health sciences biocompatibility 0302 clinical medicine mta Media Technology Medicine General Materials Science Control material Marketing mtt business.industry RK1-715 030206 dentistry calcium silicates 021001 nanoscience & nanotechnology In vitro biocompatibility Distilled water Dentistry 0210 nano-technology business Apical papilla biomaterials Nuclear chemistry |
| Zdroj: | Balkan Journal of Dental Medicine, Vol 21, Iss 3, Pp 167-170 (2017) Balkan Journal of Dental Medicine |
| ISSN: | 2335-0245 |
| Popis: | Background/Aim: Lately, fully innovative sol-gel method with high-temperature self-propagating reaction was used for the synthesis of new nanostructured endodontic materials, in combination with different radiopacifiers: bismuth (ALBO-MPCA1) and barium (ALBO-MPCA2). The aim of this study was to investigate the biocompatibility of nanostructured endodontic materials based on highly active calcium silicates and mixed with different radiopacifiers in comparison to MTA+ using human stem cells from the apical papilla- SCAP cells. Material and Methods: Morphology of the samples was studied by SEM. The tested materials were mixed with distilled water in a ratio 2:1 (m/m). Fifteen minutes after the preparation, samples were used in the experiment. The biocompatibility of fresh materials, after 3h and 7 days, was tested using 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide- MTT test. Results: Samples mostly consisted of spherical and rode-like. The relative viability of cells increased following the exposure time. Conclusion: The biocompatibility of synthesized materials is comparable to the control material MTA+, and therefore these materials can be recommended for further clinical studies. |
| Databáze: | OpenAIRE |
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