Serological survey of antibodies against SARS‐CoV‐2 in dogs and cats, Thailand

Autor: Navapon Techakriengkrai, Napawan Bunpapong, Supanat Boonyapisitsopa, Kitikhun Udom, Kamonpan Charoenkul, Ekkapat Chamsai, Waleemas Jairak, Alongkorn Amonsin
Rok vydání: 2021
Předmět:
Zdroj: Transboundary and Emerging Diseases
ISSN: 1865-1682
1865-1674
Popis: Coronavirus disease of 2019 (COVID‐19) caused by severe acute respiratory syndrome virus type 2 (SARS‐CoV‐2) is an emerging severe acute respiratory disease affecting global human health. In this study, a large‐scale serological survey of antibodies against SARS‐CoV‐2 in dogs and cats was conducted during the first and second waves of COVID‐19 outbreaks in Thailand, from April to December 2020. A total of 3215 serum samples were collected from dogs (n = 2102) and cats (n = 1113) living in Bangkok and in the vicinities. Serum samples were tested for SARS‐CoV‐2 antibodies by using an indirect multispecies enzyme‐linked immunosorbent assay (ELISA). Positive and suspected samples were additionally tested for neutralizing antibodies by the surrogate virus neutralization test (sVNT). The indirect ELISA results showed that 1.66% (35 out of 2103) of dogs and 0.36% (four out of 1112) of cats were positive for SARS‐CoV‐2 antibodies. The sVNT results showed that all ELISA‐positive and suspected samples were negative for neutralizing antibodies. Positive serum samples (35 dogs and four cats) were obtained from clinically healthy animals and animals with mild respiratory signs aged
Databáze: OpenAIRE