A Single Session of Aerobic Exercise Mediates Plasticity-Related Phosphorylation in both the Rat Motor Cortex and Hippocampus
| Autor: | A. Russell Tupling, Jonathan S. Thacker, W. Richard Staines, Cerise Tang, John G. Mielke, Yuyi Xu |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male Hippocampus AMPA receptor Receptors N-Methyl-D-Aspartate Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Ca2+/calmodulin-dependent protein kinase Physical Conditioning Animal Aerobic exercise Animals Receptors AMPA Phosphorylation Receptor Neuronal Plasticity Chemistry General Neuroscience Motor Cortex Tyrosine phosphorylation Rats 030104 developmental biology Synaptic plasticity Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | Neuroscience. 412 |
| ISSN: | 1873-7544 |
| Popis: | A single session of aerobic exercise may offer one means to “prime” motor regions to be more receptive to the acquisition of a motor skill; however, the mechanisms whereby this priming may occur are not clear. One possible explanation may be related to the post-translational modification of plasticity-related receptors and their associated intracellular signaling molecules, given that these proteins are integral to the development of synaptic plasticity. In particular, phosphorylation governs the biophysical properties (e.g., Ca2+ conductance) and the migratory patterns (i.e., trafficking) of plasticity-related receptors by altering the relative density of specific receptor subunits at synapses. We hypothesized that a single session of exercise would alter the subunit phosphorylation of plasticity-related receptors (AMPA receptors, NMDA receptors) and signaling molecules (PKA, CaMKII) in a manner that would serve to prime motor cortex. Young, male Sprague–Dawley rats (n = 24) were assigned to either exercise (Moderate, Exhaustion), or non-exercising (Sedentary) groups. Immediately following a single session of treadmill exercise, whole tissue homogenates were prepared from both the motor cortex and hippocampus. We observed a robust (1.2–2.0 × greater than sedentary) increase in tyrosine phosphorylation of AMPA (GluA1,2) and NMDA (GluN2A,B) receptor subunits, and a clear indication that exercise preferentially affects pPKA over pCaMKII. The changes were found, specifically, following moderate, but not maximal, acute aerobic exercise in both motor cortex and hippocampus. Given the requirement for these proteins during the early phases of plasticity induction, the possibility exists that exercise-induced priming may occur by altering the phosphorylation of plasticity-related proteins. |
| Databáze: | OpenAIRE |
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