Volume sensing in the transient receptor potential vanilloid 4 ion channel is cell type–specific and mediated by an N-terminal volume-sensing domain

Autor: David Križaj, Nanna MacAulay, Tam T. T. Phuong, Trine Lisberg Toft-Bertelsen, Oleg Yarishkin, Sarah Redmon
Rok vydání: 2019
Předmět:
0301 basic medicine
retina
transient receptor potential channels (TRP channels)
Patch-Clamp Techniques
Cell
M?ller cells
Xenopus
Gating
Biochemistry
14Z-eicosatrienoic acid (5?
Mice
Xenopus laevis
Transient receptor potential channel
transient receptor potential vanilloid 4 (TRPV4)
Neurobiology
phospholipase A2 (PLA2)
Phosphorylation
Cytoskeleton
Neurons
biology
Chemistry
volume sensor
medicine.anatomical_structure
11Z
Female
Ion Channel Gating
Neuroglia
Muller glia
6?-epoxy-8Z
TRPV4
Ependymoglial Cells
osmo-sensing
TRPV Cation Channels
03 medical and health sciences
lipid
medicine
Animals
Molecular Biology
Ion channel
Xenopus oocytes
Cell Size
aquaporin 4 (AQP4)
030102 biochemistry & molecular biology
osmo-sensor
volume sensing
cell swelling
polyunsaturated fatty acid (PUFA)
Cell Biology
biology.organism_classification
6?-EET)
Rats
Phospholipases A2
glaucoma
030104 developmental biology
retinal ganglion cells
5?
Oocytes
Biophysics
Zdroj: J Biol Chem
Toft-Bertelsen, T L, Yarishkin, O, Redmon, S, Phuong, T T T, Krizaj, D & MacAulay, N 2019, ' Volume sensing in the transient receptor potential vanilloid 4 ion channel is cell type-specific and mediated by an N-terminal volume-sensing domain ', Journal of Biological Chemistry, vol. 294, no. 48, pp. 18421-18434 . https://doi.org/10.1074/jbc.RA119.011187
ISSN: 0021-9258
Popis: Many retinal diseases are associated with pathological cell swelling, but the underlying etiology remains to be established. A key component of the volume-sensitive machinery, the transient receptor potential vanilloid 4 (TRPV4) ion channel, may represent a sensor and transducer of cell swelling, but the molecular link between the swelling and TRPV4 activation is unresolved. Here, our results from experiments using electrophysiology, cell volumetric measurements, and fluorescence imaging conducted in murine retinal cells and Xenopus oocytes indicated that cell swelling in the physiological range activated TRPV4 in Müller glia and Xenopus oocytes, but required phospholipase A(2) (PLA(2)) activity exclusively in Müller cells. Volume-dependent TRPV4 gating was independent of cytoskeletal rearrangements and phosphorylation. Our findings also revealed that TRPV4-mediated transduction of volume changes is dependent by its N terminus, more specifically by its distal-most part. We conclude that the volume sensitivity and function of TRPV4 in situ depend critically on its functional and cell type–specific interactions.
Databáze: OpenAIRE
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