Functional analysis of agalactosyl IgG in inflammatory bowel disease patients
| Autor: | Tsunekazu Mizushima, Satoshi Egawa, Tomofumi Akasaka, Masahiko Tsujii, Norio Hayashi, Tsutomu Nishida, Eiichi Morii, Hideki Iijima, Jumpei Kondo, Sachiko Nakajima, Shinichiro Shinzaki, Eiji Miyoshi, Yoshito Hayashi, Takahiro Inoue, Tatsuya Kanto, Akira Mukai |
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| Rok vydání: | 2011 |
| Předmět: |
Erythrocytes
Blotting Western Enzyme-Linked Immunosorbent Assay Nerve Tissue Proteins chemical and pharmacologic phenomena Inflammatory bowel disease Monocytes Immunoglobulin G Immunoenzyme Techniques Immune system Crohn Disease Phagocytosis Intestinal mucosa medicine Animals Humans Immunology and Allergy RNA Messenger Sheep biology Reverse Transcriptase Polymerase Chain Reaction business.industry Gastroenterology Lectin Myelin Basic Protein Dendritic Cells bacterial infections and mycoses medicine.disease Ulcerative colitis digestive system diseases Complement system Case-Control Studies Immunology biology.protein Cytokines Colitis Ulcerative Antibody business Biomarkers Transcription Factors |
| Zdroj: | Inflammatory Bowel Diseases. 17:927-936 |
| ISSN: | 1078-0998 |
| Popis: | Background: Agalactosyl immunoglobulin (Ig) G is increased in inflammatory bowel disease (IBD) similarly to rheumatoid arthritis (RA). The lectin complement pathway is shown to be activated through association of agalactosyl IgG with mannan-binding lectin (MBL) in RA. Functional changes of IgG agalactosylation in IBD, however, have not yet been clarified. Methods: The ratio of the agalactosyl/non-agalactosyl fraction in fucosylated IgG oligosaccharides (G0F/G2F) and serum MBL levels were analyzed in 59 patients with Crohn's disease (CD), 64 ulcerative colitis (UC), and 39 healthy volunteers (HV). The MBL levels associated with serum IgG were analyzed by enzyme-linked immunosorbent assay. MBL expression in the intestinal mucosa was analyzed by immunohistochemistry. Phagocytosis of sheep red blood cells (SRBC) reacted with either an agalactosyl or non-agalactosyl SRBC-specific IgG antibody was determined by flow cytometry. Results: The serum MBL levels were not significantly different among CD, UC, or HV. In patients with CD, the serum MBL levels were negatively correlated with the Crohn's Disease Activity Index (CDAI). The levels of MBL associated with agalactosyl IgG were not different from those associated with non-agalactosyl IgG. Immunoreactivity to MBL was less in the inflamed mucosa compared with the noninflamed mucosa. Phagocytic activity of SRBC was significantly higher in the presence of agalactosyl IgG compared to non-agalactosyl IgG. Conclusions: Agalactosyl IgG oligosaccharides enhanced antibody-dependent phagocytosis in vitro but did not activate the lectin complement pathway. Oligosaccharide alterations of IgG are not only a marker of IBD but also functionally modulate the immune function of IBD. (Inflamm Bowel Dis 2010;) |
| Databáze: | OpenAIRE |
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